FGFR4 ELISA Kits: Novus Biologicals

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2 Results for "fgfr4" in ELISA Kits

DYC685-2

Human Total FGFR4 DuoSet IC ELISA, 2 Plate

1 Kit (for 2 Plates)

DYC685-2

ELISA FGFR4 [Biotin]

Lysates prepared from MDA-MB-453 human breast cancer cells were serially diluted and analyzed by (A) IP-Western blot and (B) this DuoSet® IC ELISA. IPs were performed using an anti-FGF R4 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-FGF R4 monoclonal antibody to detect total FGF R4. Bands were visualized by chemiluminescent detection. Human FGF R4 can be detected by this DuoSet IC ELISA by using approximately 2 to 4 times less lysate than is needed for a conventional IP-Western blot.

Lysates were prepared from MDA-MB-453 human breast cancer cells and HEK293 human embryonic kidney cells transfected with human FGF R4 (HEK293-hFGF R4). ELISA was performed using 100 μg of the MDA-MB-453 cell lysate and 5 μg of the HEK293-hFGF R4 cell lysate. IP-Western blot analyses were performed as described in Figure 1, using 400 μg of cell lysate.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC685-5

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DYC5516-2

Human Phospho-FGFR4 DuoSet IC ELISA, 2 Plate

(1 Publication)

1 Kit (for 2 Plates)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Conjugate	Catalog #	Availability	Size	Price
Biotin	DYC5516-5

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	DYC5516-5

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