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APAF-1 Antibody (94408)

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 Monoclonal Antibody (Catalog # MAB868) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for APAF-1 was detected at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. Gels were loaded with 2 x 105cells (lane 1) and 7 x 104cells (lane 2). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human APAF-1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
94408
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB868-SP
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APAF-1 Antibody (94408) Summary

Immunogen
E. coli-derived recombinant human APAF-1
Leu10-Asp214
Accession # AAC51678
Specificity
Detects human APAF-1 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APAF1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB868 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APAF-1 Antibody (94408)

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

Apoptosis protease-activating factor 1 (APAF‑1) is a ubiquitous 135 kDa protein component of the cytoplasmic apoptosome complex. APAF‑1 plays an important role in the mitochondrial apoptotic pathway by triggering the Cytochrome c-dependent activation of Caspase-9. APAF‑1 participates in tissue morphogenesis during development, and loss of APAF‑1 expression is associated with tumor progression in a variety of cancers. Some alternate splice forms of APAF‑1 are deficient in their ability to promote apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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APAF-1 Antibody
(94408)
MAB868
APAF-1 Antibody (94408)
Species: Hu
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Publications for APAF-1 Antibody (MAB868)(8)

We have publications tested in 3 confirmed species: Human, Rat, Chicken.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(1)
Western Blot
(7)
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Human
(5)
Rat
(2)
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(1)
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Showing Publications 1 - 8 of 8.
Publications using MAB868 Applications Species
AJ Rodrigues, JJ Bandolik, FK Hansen, T Kurz, A Hamacher, MU Kassack Priming with HDAC Inhibitors Sensitizes Ovarian Cancer Cells to Treatment with Cisplatin and HSP90 Inhibitors Int J Mol Sci, 2020-11-05;21(21):. 2020-11-05 [PMID: 33167494] (Western Blot, Human) Western Blot Human
HL Hayes, BS Peterson, JM Haldeman, CB Newgard, HE Hohmeier, SB Stephens Delayed apoptosis allows islet ?-cells to implement an autophagic mechanism to promote cell survival PLoS ONE, 2017-02-17;12(2):e0172567. 2017-02-17 [PMID: 28212395] (Western Blot, Rat) Western Blot Rat
Mungamuri S, Wang S, Manfredi J, Gu W, Aaronson S Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene, 2014-07-14;34(19):2461-70. 2014-07-14 [PMID: 25023704] (Western Blot, Human) Western Blot Human
Kracikova , M, Akiri , G, George , A, Sachidanandam , R, Aaronson , S A A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ, 2013-01-11;20(4):576-88. 2013-01-11 [PMID: 23306555] (Western Blot, Human) Western Blot Human
Bano D, Dinsdale D, Cabrera-Socorro A, Maida S, Lambacher N, McColl B, Ferrando-May E, Hengartner MO, Nicotera P Alteration of the nuclear pore complex in Ca(2+)-mediated cell death. Cell Death Differ., 2010-01-01;17(1):119-33. 2010-01-01 [PMID: 19713973] (ICC, Rat) ICC Rat
Karki P, Seong C, Kim JE, Hur K, Shin SY, Lee JS, Cho B, Park IS Intracellular K(+) inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release. Cell Death Differ., 2007-09-21;14(12):2068-75. 2007-09-21 [PMID: 17885667] (Western Blot, Human) Western Blot Human
Sato T, Machida T, Takahashi S, Iyama S, Sato Y, Kuribayashi K, Takada K, Oku T, Kawano Y, Okamoto T, Takimoto R, Matsunaga T, Takayama T, Takahashi M, Kato J, Niitsu Y Fas-mediated apoptosome formation is dependent on reactive oxygen species derived from mitochondrial permeability transition in Jurkat cells. J. Immunol., 2004-07-01;173(1):285-96. 2004-07-01 [PMID: 15210786] (Western Blot, Human) Western Blot Human
Sanders EJ, Parker E The role of mitochondria, cytochrome c and caspase-9 in embryonic lens fibre cell denucleation. J. Anat., 2002-08-01;201(2):121-35. 2002-08-01 [PMID: 12220121] (Western Blot, Chicken) Western Blot Chicken

Reviews for APAF-1 Antibody (MAB868) (0)

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Bioinformatics

Gene Symbol APAF1
Uniprot