The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating both nuclear and mitochondrial gene transcription. The complex also includes pol II, and transcription factors TFIIA-F. It serves as a central scaffold within the pre-initiation complex (PIC) responsible for converting biological stimuli (in the form of transcription factors) into gene expression responses.
