Base Excision Repair

By Natalia Gurule, PhD

Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and response of cells to stress.

The PARP (Poly(ADP-ribose) polymerase) protein is a zinc-dependant nuclear enzyme whose main role is to detect and repair DNA single-strand breaks (SSB). However, PARP antibody research has revealed there are at least 17 PARP proteins, which also play a major role in apoptosis (programmed cell death) and post-translational modification. We at Novus Biologicals have an extensive range of PARP antibody products in our antibody catalogu.

Base excision repair (BER) is the most fundamental DNA repair mechanism, dealing with alterations arising in individual DNA bases during cellular metabolism. We at Novus Biologicals have a large BER antibody database, which has proven important in various cellular studies.

A large number of antibody assays are devoted to the study of nuclear excision repair (NER) proteins. However, there are a number of other DNA repair pathways, many of which are instigated by NER and share the same proteins. DNA repair antibodies are widely used in cancer research, and Novus Biologicals offers an extensive selection of thoroughly validated, high-quality DNA repair and cancer related antibodies.

The mammalian DNA repair process encompasses a large number of protein pathways, and forms an important part of our antibody catalog. The past 30 years have seen tremendous advances in the understanding of these mechanisms, with Wood, et al. recently compiling an updated inventory of at least 150 human DNA-repair proteins. This added considerably to the scope of the antibody database of us at Novus Biologicals.