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Automated Antibody Immunoprecipitation with the Precipitor - Saving Time and Money

Fri, 08/19/2011 - 11:25


We at Novus Biologicals have a large catalogue of protein, peptide and antibody products to choose from. To make life easier for researchers, our catalog includes products like the actin antibody pack, which contains two actin antibody vials mapped to the N-termini of human beta and gamma actin.

Antibody packs contain multiple vials of similarly targeted immunoglobulins, which researchers can test to find the best product for their assay. Buying them in kit form saves the tedium of hunting through the catalog to match up likely products (of which there are over 500 for the actin antibody alone). However, the process can be improved even further with the PrecipitorTM Affinity Magnetic Precipitation System.

Precipitor

PrecipitorTM is an automated, high-throughput automated system which uses affinity conjugated magnetic beads to precipitate and purify proteins. Chromatin Immunoprecipitation (ChIP) and recombinant protein purification are just two of many possible applications. The 96 deep-well platform easily accommodates 16 simultaneous assays, via a robotic, system of parallel magnetic rods. This makes PrecipitorTM results highly specific, reproducible and consistent, and eliminates all the drawbacks associated with manual assays, including antibody waste.

It is easy to see the benefits of using PrecipitorTM by looking at a typical beta actin antibody assay. Expressed in all cells throughout the body, beta actin is a common loading control in Western blotting and similar protein detection assays. However, even a common beta actin antibody can prove problematic, and you may need to perform several tests to determine the best ratio of antibody to protein, for example. The PrecipitorTM minimizes problem by simplifying and streamlining what is typically a routine, but labour-intensive process.


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