Immunogen | Human C-terminal peptide (aa. 475-490). |
Localization | Secreted. |
Specificity | This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs. |
Isotype | IgG |
Clonality | Polyclonal |
Host | Rabbit |
Gene | MMP2 |
Purity | Immunogen affinity purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | This MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 28987763). Use in ELISA reported in scientific literature (PMID: 8299723). Use in electron microscopy reported in scientific literature (PMID: 24905939) |
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Theoretical MW | 70 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Immunogen affinity purified |
Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Verified Customer |
IF | Human | 06/07/2018 |
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reviewed by:
Guo Mengqi |
WB | Mouse | 01/23/2018 |
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reviewed by:
Guo Mengqi |
IHC-Fr | Mouse | 01/23/2018 |
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reviewed by:
Verified Customer |
Simple Western | Mouse | 08/16/2017 |
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reviewed by:
Ruhul Choudhury |
IHC-P | Human | 01/18/2017 |
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reviewed by:
Verified Customer |
WB | Mouse | 12/23/2014 |
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reviewed by:
Verified Customer |
IF | Human | 11/20/2014 |
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Secondary Antibodies |
Isotype Controls |
Research Areas for MMP-2 Antibody (NB200-193)Find related products by research area.
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MMP-2: More Than a Cancer Marker Matrix metalloproteinases (MMP) are a family of endopeptidases involved in the breakdown of extracellular matrix (ECM) during both normal physiological and disease processes. MMP-2 is a zinc-dependent family member that selectively cleaves collagen... Read full blog post. |
CD63: is it pro-metastatic or anti-metastatic? CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,... Read full blog post. |
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Verified Customer 06/07/2018 |
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Application: | IF | |
Species: | Human |
Guo Mengqi 01/23/2018 |
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Application: | WB | |
Species: | Mouse |
Guo Mengqi 01/23/2018 |
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Application: | IHC-Fr | |
Species: | Mouse |
Gene Symbol | MMP2 |