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MMP-2 Antibody - (Pro and Active)

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Summary
Product Discontinued
View other related MMP-2 Primary Antibodies

Order Details


    • Catalog Number
      NB200-193
    • Availability
      Product Discontinued

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MMP-2 Antibody - (Pro and Active) Summary

Immunogen
Human C-terminal peptide (aa. 475-490).
Localization
Secreted.
Specificity
This antibody is specific for pro and active forms of MMP2. It was tested in WB againsst recombinant mouse MMP-2, MMP3, MMP7, MMP9, and MMP16 wherein it did not react with other MMPs.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Electron Microscopy
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:500-1:1000
  • Immunohistochemistry 1:500-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Simple Western
  • Western Blot 1:10000
Application Notes
This MMP2 antibody is useful for Western Blot, Immunocytochemistry/Immunofluoresence, and Immunohistochemistry on frozen and paraffin-embedded sections. In Western Blot, bands can be seen at approximately 72kDa (proform) and 62kDa (active form). Antigen retrieval is not required for IHC. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW reported in scientific literature (PMID: 28987763). Use in ELISA reported in scientific literature (PMID: 8299723). Use in electron microscopy reported in scientific literature (PMID: 24905939)
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB200-193 in the following applications:

Publications
Read Publications using
NB200-193 in the following applications:

Reactivity Notes

Guinea Pig reactivity reported in scientific literature (PMID: 26982283).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MMP-2 Antibody - (Pro and Active)

  • 72 kDa gelatinase
  • CLG4
  • CLG4A72 kDa type IV collagenase
  • collagenase type IV-A
  • EC 3.4.24
  • EC 3.4.24.24
  • Gelatinase A
  • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
  • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
  • Matrix metalloproteinase-2
  • matrix metalloproteinase-II
  • MMP-2 ihc
  • MMP-2 mouse
  • MMP-2 western blot
  • MMP2
  • MMP-2
  • MMP-II
  • MONA
  • neutrophil gelatinase
  • TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

MMP2 (Matrix metalloproteinase-2, gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), is a member of the matrix metalloproteinase (MMP) enzyme family. Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMP-2 Antibody (NB200-193)(57)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig, Rabbit.

We have publications tested in 9 applications: CostimT, ELISA, EM, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.


Filter By Application
CostimT
(1)
ELISA
(2)
EM
(1)
FLOW
(1)
ICC/IF
(1)
IF/IHC
(4)
IHC-Fr
(3)
IHC-P
(10)
WB
(26)
All Applications
Filter By Species
Human
(10)
Mouse
(20)
Rat
(11)
Chicken
(2)
Guinea Pig
(2)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using NB200-193 Applications Species
Lin C, Huang P, Chen C et al. Tributyrin Intake Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm in LDLR-/- Mice International Journal of Molecular Sciences 2023-04-28 [PMID: 37175712]
Valerio V, Keceli G, Moschetta D et al. Enduring ROS emission causes aberrant protein S-glutathionylation transitioning human aortic valve cells from a sclerotic to a stenotic phenotype Antioxidants & redox signaling [PMID: 35459416]
She M, Li B, Li T et al. Modulation of the ERK1/2 MMP 2 pathway in the sclera of guinea pigs following induction of myopia by flickering light Experimental and Therapeutic Medicine 2021-02-19 [PMID: 33732344] (WB, IHC-P, Guinea Pig) WB, IHC-P Guinea Pig
Aversano, A, Rossi, F W Et al. Nitrodi thermal water downregulates protein S nitrosylation in RKO cells. Int J Mol Med 2020-10-01 [PMID: 32945437] (IF/IHC, Mouse) IF/IHC Mouse
Wen H, Wang M, Gong S et al. Human Umbilical Cord Mesenchymal Stem Cells Attenuate Abdominal Aortic Aneurysm Progression in Sprague Dawley Rats: Implication of Vascular Smooth Muscle Cell Phenotypic Modulation Stem Cells Dev. 2020-06-03 [PMID: 32486904]
Xue Q, Wang X, Deng X Et Al. CEMIP regulates the proliferation and migration of vascular smooth muscle cells in atherosclerosis through the WNT-beta-catenin signaling pathway Biochem. Cell Biol. 2020-03-24 [PMID: 32207314]
Retana-Lobo C, Guerreiro-Tanomaru Jm, Tanomaru-Filho M et Al. Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin Materials (Basel) 2020-02-27 [PMID: 32120926] (IHC-P, Human) IHC-P Human
Weng CL, Chen CC, Tsou HH, et al. Areca nut procyanidins prevent ultraviolet light B-induced photoaging via suppression of cyclooxygenase-2 and matrix metalloproteinases in mouse skin Drug Chem Toxicol 2019-12-02 [PMID: 31790610] (WB, Mouse) WB Mouse
Zhang C, He X, Murphy SR et al. Knockout of Dual-Specificity Protein Phosphatase 5 Protects Against Hypertension-induced Renal Injury J. Pharmacol. Exp. Ther. 2019-05-22 [PMID: 31118214] (Rat) Rat
Pilz PM, Hamza O, Gidlof O et al. Remote ischemic perconditioning attenuates adverse cardiac remodeling and preserves left ventricular function in a rat model of reperfused myocardial infarction Int. J. Cardiol. 2019-03-06 [PMID: 30904281] (IHC-P, Rat) IHC-P Rat
Show All 57 Publications.

Reviews for MMP-2 Antibody (NB200-193) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 2 species: Human, Mouse.

Reviews using NB200-193:
Filter by Applications
IF
(2)
WB
(2)
IHC-Fr
(1)
Simple Western
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(3)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence MMP-2 NB200-193
Enlarge
5
reviewed by:
Verified Customer
IF Human 06/07/2018
View

Summary

ApplicationImmunofluorescence
Sample Testedfibroblasts
SpeciesHuman
Lot022013
Western Blot MMP-2 NB200-193
Enlarge
5
reviewed by:
Guo Mengqi
WB Mouse 01/23/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse vascular smooth muscle cell
SpeciesMouse
LotLot022013
Immunohistochemistry-Frozen MMP-2 NB200-193
Enlarge
5
reviewed by:
Guo Mengqi
IHC-Fr Mouse 01/23/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse aortic root
SpeciesMouse
LotLot022013
Simple Western MMP-2 NB200-193
Enlarge
3
reviewed by:
Verified Customer
Simple Western Mouse 08/16/2017
View

Summary

ApplicationSimple Western
Sample Testedtail artery lysate
SpeciesMouse
Lot0910-03

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with a rabbit detection system. Lysis buffer: RIPA, prot. conc.: 0.5 µg/µl.
Immunohistochemistry-Paraffin MMP-2 NB200-193
Enlarge
4
reviewed by:
Ruhul Choudhury
IHC-P Human 01/18/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman decidua
SpeciesHuman
Lot0213-02

Comments

CommentsSodium Citrate antigen retrieval.
  5
reviewed by:
Verified Customer
WB Mouse 12/23/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24281761
SpeciesMouse
Immunofluorescence MMP-2 NB200-193
Enlarge
4
reviewed by:
Verified Customer
IF Human 11/20/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
Lot21512

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MMP-2 Antibody (NB200-193). (Showing 1 - 1 of 1 FAQs).

  1. Is this MMP2 antibody is good for mouse tissue? Are there any publications that have a mouse model using this antibody? Also does it need any antigen retrieval to do paraffin sections?
    • NB200-193 has been validated and is guaranteed for use in human, mouse, rat and chicken samples in Western blot, flow cytometry, immunocytochemistry, and immunohistochemistry in both frozen and paraffin-embedded sections. Antigen retrieval is recommended for using this antibody on paraffin-embedded sections.

Secondary Antibodies

 

Isotype Controls

Additional MMP-2 Products

Research Areas for MMP-2 Antibody (NB200-193)

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Blogs on MMP-2.

MMP-2: More Than a Cancer Marker
Matrix metalloproteinases (MMP) are a family of endopeptidases involved in the breakdown of extracellular matrix (ECM) during both normal physiological and disease processes. MMP-2 is a zinc-dependent family member that selectively cleaves collagen...  Read full blog post.

CD63: is it pro-metastatic or anti-metastatic?
CD63 is a type II membrane protein belonging to tetraspanin superfamily and it play key roles in the activation of several cellular signaling cascades along with acting as TIMP1 receptor. It is expressed by activated platelets, monocytes,...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Verified Customer
06/07/2018
Application: IF
Species: Human

Guo Mengqi
01/23/2018
Application: WB
Species: Mouse

Guo Mengqi
01/23/2018
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MMP2