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![Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot using lysates from U20S parental cell line and Dynamin knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Dynamin Monoclonal Antibody (Catalog # NB110-60491) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Dynamin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Simple Western: Dynamin Antibody (3G4B6) [NB110-60491] - Simple Western lane view shows a specific band for Dynamin in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Simple-Western-NB110-60491-img0012.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using Dynamin mouse mAb against C6 (1), NIH/3T3 (2), SKN-SH (3), LN18 (4), SHSY5Y (5) cell lysate and rat brain tiisues lysate (6).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0003.jpg)
![Immunoprecipitation: Dynamin Antibody (3G4B6) [NB110-60491] - Antibody-bead conjugates were prepared by adding 1.0 ug of antibody to 500 ul of PBS with 0,01% triton X-100 in a microcentrifuge tube and 30ul of protein G Sepharose beads. Tubes were rocked O/N at 4 degrees C followed by several washes to remove unbound antibodies. U2OS were collected in HEPES buffer (20 mM HEPES, 100 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, pH 7.4) supplemented with protease inhibitor. Lysates are rocked 30 min at 4 degrees C and spun at 110,000xg for 15 min at 4 degrees C. One ml aliquots at 1 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4 degrees C. Following centrifugation, the unbound fractions were collected, and beads were subsequently washed three times with 1 ml of HEPES lysis buffer and processed for SDS-PAGE and immunoblot. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunoprecipitation-NB110-60491-img0014.jpg)
![Western Blot: Dynamin Antibody (3G4B6) [NB110-60491] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Western-Blot-NB110-60491-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody (3G4B6) [NB110-60491] - Immunohistochemical analysis of paraffin-embedded human Lymph tissue, glioma tissue and cerebellum tissue, showing membrane localization using Dynamin antibody with DAB staining.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Immunohistochemistry-Paraffin-NB110-60491-img0004.jpg)
![Flow Cytometry: Dynamin Antibody (3G4B6) [NB110-60491] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Dynamin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Flow-Cytometry-NB110-60491-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: Dynamin Antibody (3G4B6) [NB110-60491] - U2OS WT and DNM1 KO cells were labelled with a green or a far red fluorescence dye, respectively. WT and KO cells were mixed and plated to a 1 :1 ratio on coverslips. Cells were stained with and with the corresponding Alexa-fluor 555 coupled secondary antibody. Acquisition of the green (WT), red (antibody staining) and far-red (KO) channels was performed. Representative grayscale image of the red channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Antibody dilution used: 1/1000. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Dynamin-Antibody-3G4B6-Knockout-Validated-NB110-60491-img0015.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody [NBP2-48950] - Staining in human cerebral cortex and kidney tissues using anti-DNM1 antibody. Corresponding DNM1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Immunohistochemistry-Paraffin-NBP2-48950-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dynamin Antibody [NBP2-48950] - Staining in human cerebral cortex and kidney tissues using anti-DNM1 antibody. Corresponding DNM1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Dynamin Antibody [NBP2-48950] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48950-img0005.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody [NBP2-48950] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Immunohistochemistry-Paraffin-NBP2-48950-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin Antibody [NBP2-48950] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Immunohistochemistry-Paraffin-NBP2-48950-img0003.jpg)
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0003.jpg "Western Blot: Dynamin Antibody (D5) [NB100-1993] - Analysis of chicken SL29 cell Lysate")
![Western Blot: Dynamin Antibody (D5) [NB100-1993] - Western blot analysis of Dynamin, mAb (D5): Lane 1: MW marker, Lane 2: Mouse Brain, Lane 3: Rat Brain](http://images.novusbio.com/fullsize/Dynamin-Antibody-D5-Western-Blot-NB100-1993-img0001.jpg)
![Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/Dynamin-[p-Ser774]-Antibody-Western-Blot-NB300-209-img0002.jpg "Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.](http://images.novusbio.com/fullsize/Dynamin-[p-Ser778]-Antibody-Western-Blot-NB300-210-img0001.jpg "Western Blot: Dynamin [p Ser778] Antibody [NB300-210] - Analysis of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment.")
![Western Blot: Dynamin Antibody (SR2307) [NBP3-21813] - Western blot analysis using NBP3-21813 in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21813_rabbit-dynamin-mab-sr2307-6720239533784.jpg "Western Blot: Dynamin Antibody (SR2307) [NBP3-21813] - Western blot analysis using NBP3-21813 in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate.")
![Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-27820241631653.jpg "Western Blot: Dynamin Antibody (A1B2-R) [NBP3-32283] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 17 seconds; Lane 3-4: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dynamin Antibody (A1B2-R) [NBP3-32283] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Dynamin antibody (NBP3-32283) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32283) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32283_mouse-dynamin-mab-a1b2-r-298202414304193.jpg)
![Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0002.jpg "Western Blot: Dynamin Antibody [NB100-61094] - Western Blot of rat cortex lysate using NB100-61094 at 1:1000.")
![Western Blot: Dynamin Antibody [NB100-61094] - Rat cortex lysate showing labeling of the ~95k Dynamin protein.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB100-61094-img0001.jpg)
![Western Blot: Dynamin Antibody [NB300-565] - Analysis of rat brain homogenate.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Western-Blot-NB300-565-img0003.jpg "Western Blot: Dynamin Antibody [NB300-565] - Analysis of rat brain homogenate.")
![Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32282) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32282_mouse-dynamin-mab-a1b1-r-278202416285218.jpg "Western Blot: Dynamin Antibody (A1B1-R) [NBP3-32282] - Western blot analysis of Dynamin on different lysates with Mouse anti-Dynamin antibody (NBP3-32282) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 97 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32282) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Dynamin Antibody [NBP2-76558] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76558-img0001.jpg "Immunocytochemistry/Immunofluorescence: Dynamin Antibody [NBP2-76558] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.")
![ELISA: Human Dynamin ELISA Kit (Colorimetric) [NBP3-31466] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31466_human-dynamin-elisa-kit-colorimetric-206202415294092.png "ELISA: Human Dynamin ELISA Kit (Colorimetric) [NBP3-31466] - Standard Curve Reference")