| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27399 | |||
| Azide and BSA Free | NBP2-27399SS | |||
| BSA Free | NB100-56749SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51602-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70135 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7419-SP | |||
![Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-91220241519579.jpg "Immunohistochemistry: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AKT1/2 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Western Blot: AKT1/2 Antibody (1G0K5) [NBP3-33526] - Western blot analysis of various lysates using AKT1/2 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33526_rabbit-akt1-2-mab-1g0k5-912202415952.jpg)
![Western Blot: AKT1/2 Antibody (SR2005) [NBP3-27692] - Western blot analysis of AKT1/2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.](http://images.novusbio.com/fullsize/nbp3-27692_rabbit-akt1-2-mab-sr2005-75202410133496.jpg "Western Blot: AKT1/2 Antibody (SR2005) [NBP3-27692] - Western blot analysis of AKT1/2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.")
![Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231526123.jpg "Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.")
![Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-57202317161873.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231558486.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22614_rabbit-akt1-2-3-mab-sr2003-57202317215156.jpg "Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.")
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-2722024847194.jpg "Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.")
![Western Blot: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Positive Western Blot detected in: SH-SY5Y whole cell lysate. All lanes: AKT1/2/3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-282202411442636.jpg)
![Flow Cytometry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Overlay Peak curve showing Jurkat cells stained with AKT1/2/3 Antibody (16E10) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-28220241418367.jpg)
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0014.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)
![Immunohistochemistry-Frozen: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Rat mammary carcinoma tissue section stained with AKT1 [p Ser473] Antibody (104A282). IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Frozen-NB100-56749-img0016.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of pSer473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Some cancer cells depicted nuclear stianing also. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/AKT1-p-Ser473-Antibody-104A282-Immunohistochemistry-Paraffin-NB100-56749-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0006.jpg "Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis using AKT1 antibody against NIH/3T3 (1), Hela (2),COS7 (3) and Jurkat (4) cell lysate.")
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human breast carcinoma using AKT1 antibody at 1:50. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0010.jpg)
![Western Blot: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of extracts from COS cells using AKT1 antibody at 1:1000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Western-Blot-NBP1-51602-img0008.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (3A3) [NBP1-51602] - Analysis of human endometrial cancer using AKT1 antibody at 1:25. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Akt1-Antibody-3A3-Immunohistochemistry-Paraffin-NBP1-51602-img0009.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0009.jpg "Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (35 ug) from 9 different cell lines using AKT1 antibody.")
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB detection of AKT1 in human MD-MB-231 cells using AKT1 antibody NBP2-01724 at a dilution of 1:2000. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 Antibody (OTI4D6) [NBP2-01724] - ICC/IF of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunocytochemistry-Immunofluorescence-NBP2-01724-img0008.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - WB analysis of extracts (10 ug) from a mouse cell line and 3 different mouse tissues using AKT1 antibody at a 1:200 dilution.](http://images.novusbio.com/fullsize/Akt1-Antibody-4D6-Western-Blot-NBP2-01724-img0012.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human prostate tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0007.jpg)
![Flow Cytometry: AKT1 Antibody (OTI4D6) [NBP2-01724] - Flow cytometric analysis of Jurkat cells using anti-AKT1 antibody (red), compared to a nonspecific negative control antibody (blue).](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Flow-Cytometry-NBP2-01724-img0001.jpg)
![Western Blot: AKT1 Antibody (OTI4D6) [NBP2-01724] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY AKT1 (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT1.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Western-Blot-NBP2-01724-img0002.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded adenocarcinoma of human endometrium tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0003.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human bladder tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0004.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded carcinoma of human kidney tissue using AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0005.jpg)
![Immunohistochemistry-Paraffin: AKT1 Antibody (OTI4D6) [NBP2-01724] - IHC-P staining of paraffin-embedded human kidney tissue using anti-AKT1 antibody. Heat-induced epitope retrieval in 10 mM citric buffer, pH 6.0, 100C for 10 min.](http://images.novusbio.com/fullsize/AKT1-Antibody-4D6-Immunohistochemistry-Paraffin-NBP2-01724-img0006.jpg)
