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![Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Western-Blot-NBP2-67381-img0008.jpg "Western Blot: MLH1 Antibody (SP08-04) [NBP2-67381] - Western blot analysis of MLH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (SP08-04) [NBP2-67381] - Staining MLH1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunocytochemistry-Immunofluorescence-NBP2-67381-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0007.jpg)
![Flow Cytometry: MLH1 Antibody (SP08-04) [NBP2-67381] - Analysis of Hela cells with MLH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Flow-Cytometry-NBP2-67381-img0001.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0005.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (SP08-04) [NBP2-67381] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MLH1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Immunohistochemistry-Paraffin-NBP2-67381-img0006.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: MLH1 Antibody (SP08-04) [NBP2-67381] - All lanes: Western blot analysis of MLH1 with anti-MLH1 antibody [SP08-04] at 1:1,000 dilution. Lane 1: Wild-type B16F10 whole cell lysate. Lane 2: MLH1 knockout B16F10 whole cell lysate. NBP2-67381 was shown to specifically react with MLH1 in wild-type B16F10 cells. No band was observed when MLH1 knockout samples were tested. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MLH1 antibody (1/1,000) and Anti-Vinculin antibody (1/5,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/MLH1-Antibody-SP08-04-Knockout-Validated-NBP2-67381-img0010.jpg)
![Western Blot: MLH1 Antibody (164C819) [NB100-56552] - Analysis of MLH1 in Daudi cell lysate using this antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/MLH1-Antibody-164C819-Western-Blot-NB100-56552-img0002.jpg "Western Blot: MLH1 Antibody (164C819) [NB100-56552] - Analysis of MLH1 in Daudi cell lysate using this antibody at 2 ug/ml.")
![Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Western blot analysis of Jurkat cell lysate using MLH1 antibody (MLH1/6284R).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Western-Blot-NBP3-13971-img0001.jpg "Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Western blot analysis of Jurkat cell lysate using MLH1 antibody (MLH1/6284R).")
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MLH1 antibody (MLH1/6284R).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0003.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - MLH1 antibody (MLH1/6284R). MLH1 Recombinant Rabbit Monoclonal (MLH1/6284R). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0002.jpg)
![Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - FFPE human Hereditary Nonpolyposis Colorectal Cancer (HNPCC) / Lynch Syndrome. MLH1 antibody (MLH1/6284R) (2ug/ml).](http://images.novusbio.com/fullsize/MLH1-Antibody-MLH1-6284R-Immunohistochemistry-Paraffin-NBP3-13971-img0004.jpg)
![Western Blot: MLH1 Antibody (S02-2E8) [NBP3-19527] - Western blot detection of MLH1 in K562, C6, 3T3, Hela cell lysates using NBP3-19527 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/MLH1-Antibody-S02-2E8-Western-Blot-NBP3-19527-img0002.jpg "Western Blot: MLH1 Antibody (S02-2E8) [NBP3-19527] - Western blot detection of MLH1 in K562, C6, 3T3, Hela cell lysates using NBP3-19527 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S02-2E8) [NBP3-19527] - Immunofluorescence of MLH1 in Hela cells using NBP3-19527 at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/MLH1-Antibody-S02-2E8-Immunocytochemistry-Immunofluorescence-NBP3-19527-img0001.jpg)
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414161884.jpg "Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunofluorescence analysis of paraffin-embedded human colon cancer tissue labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32600, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32600) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-298202414333686.jpg)
![Immunocytochemistry/ Immunofluorescence: MLH1 Antibody (PSH01-74) [NBP3-32600] - Immunocytochemistry analysis of A431 cells labeling MLH1 with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MLH1 antibody (NBP3-32600) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32600_rabbit-mlh1-mab-psh01-74-292024111485.jpg)
![Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-278202416285244.jpg "Western Blot: MLH1 Antibody (PD01-61) [NBP3-32599] - Western blot analysis of MLH1 on K562 cell lysates with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32599) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: MLH1 Antibody (PD01-61) [NBP3-32599] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MLH1 antibody (NBP3-32599) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32599) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32599_rabbit-mlh1-mab-pd01-61-298202413585516.jpg)
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody [NBP2-38677] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MLH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38677-img0003.jpg "Immunocytochemistry/Immunofluorescence: MLH1 Antibody [NBP2-38677] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: MLH1 Antibody [NBP2-38677] - Staining of human tonsil shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/MLH1-Antibody-Immunohistochemistry-NBP2-38677-img0002.jpg)
![Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/MLH1-Antibody-S07-5D3-Western-Blot-NBP3-14898-img0001.jpg "Western Blot: MLH1 Antibody (S07-5D3) [NBP3-14898] - Western blot detection of MLH1 in Hela, A549, HL-60, U2OS cell lysates using MLH1 Rabbit mAb (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![Immunocytochemistry/Immunofluorescence: MLH1 Antibody (S07-5D3) [NBP3-14898] - Immunocytochemistry of MLH1 (green) in hela using MLH1 Rabbit mAb at dilution 1/50, and DAPI (blue)](http://images.novusbio.com/fullsize/MLH1-Antibody-S07-5D3-Immunocytochemistry-Immunofluorescence-NBP3-14898-img0002.jpg)
![Western Blot: MLH1 Antibody (S05-1G4) [NBP3-19694] - Western blot detection of MLH1 in Rat Brain lysates using NBP3-19694 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.](http://images.novusbio.com/fullsize/MLH1-Antibody-S05-1G4-Western-Blot-NBP3-19694-img0001.jpg "Western Blot: MLH1 Antibody (S05-1G4) [NBP3-19694] - Western blot detection of MLH1 in Rat Brain lysates using NBP3-19694 (1:1000 diluted). Predicted band size: 85kDa. Observed band size: 85kDa.")
![SDS-Page: Recombinant Human MLH1 Protein [H00004292-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004292-P01-1.jpg "SDS-Page: Recombinant Human MLH1 Protein [H00004292-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![SDS-Page: Recombinant Human MLH1 Protein [H00004292-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004292-Q01-1.jpg "SDS-Page: Recombinant Human MLH1 Protein [H00004292-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
