| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF887-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC2055A | |||
| Carrier Free | IC2055P | |||
| Carrier Free | MAB2055-SP | |||
| Carrier Free | IC2055V-100UG | |||
| Carrier Free | IC2055U-100UG | |||
| Carrier Free | IC2055T-100UG | |||
| Carrier Free | IC2055S-100UG | |||
| Carrier Free | IC2055R-100UG | |||
| Carrier Free | IC2055N-100UG | |||
| Carrier Free | IC2055G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | DYC887BE | |||
| Carrier Free | DYC887B-5 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC7794G | |||
| Carrier Free | IC7794P | |||
| Carrier Free | MAB887-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2055-SP | |||
![ELISA AKT [p Ser473] [Biotin]](http://images.novusbio.com/fullsize2/dyc887b-2_phospho-akt-s473-pan-specific-duoset-ic-elisa-5-plate-elisa-1592020122733.jpg "ELISA AKT [p Ser473] [Biotin]")
![Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-57202315545125.jpg "Immunohistochemistry: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunohistochemical analysis of paraffin-embedded human kidney, using NBP3-21578.")
![Western Blot: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Western blot analysis on 2 NIH/3T3 cell lysate treated with PDGF using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720239533735.jpg)
![Immunocytochemistry/Immunofluorescence: AKT [p Ser 473] Antibody (SR2061) [NBP3-21578] - Immunofluorescent analysis of NIH/3T3 cells treated with PDGF, using NBP3-21578.](http://images.novusbio.com/fullsize/nbp3-21578_rabbit-akt-p-ser-473-mab-sr2061-6720231051266.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0004.jpg "Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Untreated (-) and treated (+) MCF-7 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 (phospho Thr450) antibody (NBP3-13168) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0001.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0002.jpg)
![Western Blot: AKT [p Thr450] Antibody [NBP3-13168] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Thr450) antibody (NBP3-13168) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Thr450]-Antibody-Western-Blot-NBP3-13168-img0003.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:1000.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0001.jpg)
![Western Blot: AKT [p Ser473] Antibody [NBP3-13120] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT (phospho Ser473) antibody (NBP3-13120) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/AKT-[p-Ser473]-Antibody-Western-Blot-NBP3-13120-img0002.jpg)
![Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32747_rabbit-akt-p-ser473-mab-psh04-44-278202415242614.jpg "Western Blot: AKT [p Ser473] Antibody (PSH04-44) [NBP3-32747] - Western blot analysis of AKT on different lysates with Rabbit anti-AKT antibody (NBP3-32747) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 treated with 50uM LY294002 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 20 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32747) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/AKT-p-Thr308-Antibody-2O2W6-Western-Blot-NBP3-16956-img0001.jpg "Western Blot: AKT [p Thr308] Antibody (2O2W6) [NBP3-16956] - Western blot analysis of extracts of various cell lines, using Phospho-Akt-T308 antibody (NBP3-16956) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).](http://images.novusbio.com/fullsize/AKT-[p-Thr342]-Antibody-Western-Blot-NBP2-60772-img0001.jpg "Western Blot: AKT [p Thr342] Antibody [NBP2-60772] - Western blot of Drosophila lysate showing specific labelingof the ~68 kDa AKT protein phosphorylated at Thr342 in thefirst lane (-). Phosphospecificity is shown in the secondlane (+) where the immunolabeling is completely eliminatedby blot treatment with lambda phosphatase (Lambda-Ptase, 1200units for 30 minutes).")