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![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0004.jpg "Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0003.jpg)
![Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/AFM-primer-Chromatin-Immunoprecipitation-NBP1-71653-img0003.jpg "Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: AFM primer [NBP1-71653] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/AFM-primer-Chromatin-Immunoprecipitation-NBP1-71653-img0004.jpg)
![Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/alpha-Satellite-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71654-img0007.jpg "Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.")
![Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/alpha-Satellite-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71654-img0005.jpg)
![Chromatin Immunoprecipitation: alpha Satellite repeat primer [NBP1-71654] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/alpha-Satellite-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71654-img0006.jpg)
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0009.jpg "Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - 2 ug of NB21-1073 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0006.jpg)
![Chromatin Immunoprecipitation: Satellite2 repeat primer [NBP1-71655] - PCR product melt curve of 1% chromatin immunoprecipitation input sample run in duplicate.](http://images.novusbio.com/fullsize/Satellite2-repeat-primer-Chromatin-Immunoprecipitation-NBP1-71655-img0007.jpg)
![Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/MYOD1-primer-Chromatin-Immunoprecipitation-NBP1-71652-img0003.jpg "Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.")
![Chromatin Immunoprecipitation: MYOD1 primer [NBP1-71652] - 2 ug of NB21-1072 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/MYOD1-primer-Chromatin-Immunoprecipitation-NBP1-71652-img0004.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP2-19784-img0017.jpg "Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PGK1 protein stained by PGK1 antibody diluted at 1:20. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19784-img0014.jpg)
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP2-19784] - Analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19784-img0008.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein by Western blot analysis. 50 ug Rat liver lysate/extract, 10% SDS-PAGE, PGK1 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP2-19784-img0010.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: PGK1 Antibody [NBP2-19784] - PGK1 antibody detects PGK1 protein in 1% O2-treated samples by Western blot analysis. Upper panel: PGK1 antibody. Lower panel: beta Actin antibody. A: 30 ug HeLa whole cell lysate/extract (untreated). B: 30 ug HeLa whole cell lysate/extract (1% O2 treatment for 24 hours).](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP2-19784-img0011.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - 50 ug mouse brain whole cell lysate, 10% SDS PAGE, antibody at 1:1000.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP2-19784-img0013.jpg)
![Western Blot: PGK1 Antibody [NBP2-19784] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP2-19784-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PGK1 Antibody [NBP2-19784] - Analysis of methanol-fixed HeLa, using antibody at 1:50 dilution.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19784-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: PGK1 Antibody [NBP2-19784] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19784_rabbit-polyclonal-pgk1-antibody-1610202419405798.jpg)
![Western Blot: PGK1 Antibody [NBP1-33685] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP1-33685-img0007.jpg "Western Blot: PGK1 Antibody [NBP1-33685] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685] - PGK1 antibody detects PGK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. PGK1 stained by PGK1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP1-33685-img0006.jpg)
![Western Blot: PGK1 Antibody [NBP1-33685] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP1-33685-img0003.jpg)
![Western Blot: PGK1 Antibody [NBP1-33685] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:100](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP1-33685-img0004.jpg)
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-33685] - PGK1 antibody detects PGK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP1-33685-img0005.jpg)
![Western Blot: PGK1 Antibody (S04-9H9) [NBP3-14961] - Western blot detection of PGK1 in MCF7, HCT116, T47D, Hela cell lysates using PGK1 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.](http://images.novusbio.com/fullsize/PGK1-Antibody-S04-9H9-Western-Blot-NBP3-14961-img0002.jpg "Western Blot: PGK1 Antibody (S04-9H9) [NBP3-14961] - Western blot detection of PGK1 in MCF7, HCT116, T47D, Hela cell lysates using PGK1 Rabbit mAb (1:1000 diluted). Predicted band size: 45kDa. Observed band size: 45kDa.")
![Immunocytochemistry/Immunofluorescence: PGK1 Antibody (S04-9H9) [NBP3-14961] - Immunocytochemistry of PGK1 (green) in Hela cells using PGK1 Rabbit mAb at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/PGK1-Antibody-S04-9H9-Immunocytochemistry-Immunofluorescence-NBP3-14961-img0003.jpg)
![Western Blot: PGK1 Antibody (S04-9H9) [NBP3-14961] - Western blot detection of PGK1 in Hela cell lysates using PGK1 Rabbit mAb (1:1000 diluted). Predicted band size: 45KDa. Observed band size: 45KDa.](http://images.novusbio.com/fullsize/PGK1-Antibody-S04-9H9-Western-Blot-NBP3-14961-img0001.jpg)
![Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PGK1-Antibody-GT21612-Western-Blot-NBP3-13618-img0003.jpg "Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: PGK1 Antibody (GT21612) [NBP3-13618] - PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. PGK1 stained by PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PGK1-Antibody-GT21612-Immunohistochemistry-Paraffin-NBP3-13618-img0002.jpg)
![Western Blot: PGK1 Antibody (GT21612) [NBP3-13618] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (NBP3-13618) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PGK1-Antibody-GT21612-Western-Blot-NBP3-13618-img0001.jpg)
![Western Blot: PGK1 Antibody [NBP1-55154] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/PGK1-Antibody-Western-Blot-NBP1-55154-img0002.jpg "Western Blot: PGK1 Antibody [NBP1-55154] - HepG2 cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: PGK1 Antibody [NBP1-55154] - Human Intestine Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Epithelial cells of intestinal villus (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/PGK1-Antibody-Immunohistochemistry-Paraffin-NBP1-55154-img0003.jpg)