| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3754-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3316-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92847-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4216-SP | |||
![Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Intracellular distribution of Wwox (dilution 1:1000, AF6399), Dvl-1 (dilution 1:700, NBP1-58318), Dvl-2 (Dishevelled-2, dilution 1:1000,) and Dvl-3 (dilution 1:200, NBP2-24674) proteins in HET-1A and SCC-15 cell lines. Image collected and cropped by CiteAb from the following publication (www.jcancer.org/v11p4030.htm) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP1-32388-img0007.jpg "Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Intracellular distribution of Wwox (dilution 1:1000, AF6399), Dvl-1 (dilution 1:700, NBP1-58318), Dvl-2 (Dishevelled-2, dilution 1:1000,) and Dvl-3 (dilution 1:200, NBP2-24674) proteins in HET-1A and SCC-15 cell lines. Image collected and cropped by CiteAb from the following publication (www.jcancer.org/v11p4030.htm) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388] - Paraffin-embedded lung SCC, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32388-img0004.jpg)
![Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Sample(30 ug whole cell lysate)A: MOLT4 )B: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP1-32388-img0005.jpg)
![Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Whole cell extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP1-32388-img0006.jpg)
![Immunohistochemistry: Dishevelled-2 Antibody [NBP1-32388] - cytoplasm in the photoreceptorImmunohistochemistry of flat-mounted zebrafish retina using an antibody against Dishevelled-2 antibody [N2C2], Internal (NBP1-32388) at a 1:200 dilution.](http://images.novusbio.com/fullsize/nbp1-32388_rabbit-polyclonal-dishevelled-2-antibody-9102024209144.jpg)
![Western Blot: Dishevelled-3 Antibody [NBP2-38846] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Western-Blot-NBP2-38846-img0003.jpg "Western Blot: Dishevelled-3 Antibody [NBP2-38846] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG")
![Immunocytochemistry/Immunofluorescence: Dishevelled-3 Antibody [NBP2-38846] - Staining of human cell line CACO-2 shows positivity in cytokinetic bridge and centrosome.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38846-img0001.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP2-38846] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP2-38846-img0007.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP2-38846] - Staining of human fallopian tube shows moderate positivity in glandular cells.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP2-38846-img0005.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP2-38846] - Staining of human small intestine shows moderate positivity in glandular cells.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP2-38846-img0006.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP2-38846] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP2-38846-img0008.jpg)
![Western Blot: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Western blot analysis of Dishevelled-2 on different lysates with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: NCI-H1299 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 90 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32269) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-2782024163169.jpg "Western Blot: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Western blot analysis of Dishevelled-2 on different lysates with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: NCI-H1299 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 79 kDa Observed band size: 90 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32269) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32269) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-298202414333677.jpg)
![Immunocytochemistry/ Immunofluorescence: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Immunocytochemistry analysis of HeLa cells labeling Dishevelled-2 with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dishevelled-2 antibody (NBP3-32269) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-2920241192360.jpg)
![Flow Cytometry: Dishevelled-2 Antibody (PSH04-07) [NBP3-32269] - Flow cytometric analysis of HeLa cells labeling Dishevelled-2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32269, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32269_rabbit-dishevelled-2-mab-psh04-07-392024884683.jpg)
![Western Blot: Dishevelled-1 Antibody [NBP2-24648] - Analysis of Dishevelled-1 in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Western-Blot-NBP2-24648-img0004.jpg "Western Blot: Dishevelled-1 Antibody [NBP2-24648] - Analysis of Dishevelled-1 in Raji cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP2-16168-img0006.jpg "Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Dishevelled-2 Antibody [NBP2-16168] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16168-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP2-16168-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3].](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP2-16168-img0004.jpg)
![Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP2-16168-img0005.jpg)
![Western Blot: Dishevelled-3 Antibody [NBP3-03068] - Western blot analysis of extracts of various cell lines, using Dishevelled-3 antibody (NBP3-03068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Dishevelled-3 Antibody-Western Blot-NBP3-03068-img0005.jpg "Western Blot: Dishevelled-3 Antibody [NBP3-03068] - Western blot analysis of extracts of various cell lines, using Dishevelled-3 antibody (NBP3-03068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat ovary using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0004.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0001.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded mouse kidney using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0002.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-3 Antibody [NBP3-03068] - Immunohistochemistry of paraffin-embedded rat brain using Dishevelled-3 Rabbit pAb (NBP3-03068) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Dishevelled-3-Antibody-Immunohistochemistry-Paraffin-NBP3-03068-img0003.jpg)
![Western Blot: Dishevelled-1 Antibody [NBP2-92847] - Analysis of extracts of Mouse kidney, using Dishevelled-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Western-Blot-NBP2-92847-img0004.jpg "Western Blot: Dishevelled-1 Antibody [NBP2-92847] - Analysis of extracts of Mouse kidney, using Dishevelled-1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded rat testis using Dishevelled-1 .](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92847-img0003.jpg)
![Immunohistochemistry-Paraffin: Dishevelled-1 Antibody [NBP2-92847] - Paraffin-embedded mouse testis using Dishevelled-1 .](http://images.novusbio.com/fullsize/Dishevelled-1-Antibody-Immunohistochemistry-Paraffin-NBP2-92847-img0002.jpg)
![Western Blot: Dishevelled-3 Antibody [NBP3-41919] - Sample: Recombinant Dishevelled-3, Human.](http://images.novusbio.com/fullsize/nbp3-41919_rabbit-dishevelled-3-pab-612202421175965.jpg "Western Blot: Dishevelled-3 Antibody [NBP3-41919] - Sample: Recombinant Dishevelled-3, Human.")
![Western Blot: Dishevelled-3 Antibody [NBP3-41919] - Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Liver Tissue.](http://images.novusbio.com/fullsize/nbp3-41919_rabbit-dishevelled-3-pab-61220242055443.jpg)
![Western Blot: Dishevelled 2 Antibody [NBP1-58849] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Western-Blot-NBP1-58849-img0004.jpg "Western Blot: Dishevelled 2 Antibody [NBP1-58849] - Titration: 0.2-1 ug/ml, Positive Control: Transfected 293T.")
![Immunohistochemistry-Paraffin: Dishevelled 2 Antibody [NBP1-58849] - Human Small intestine, muscularis propria, 5 ug/ml.](http://images.novusbio.com/fullsize/Dishevelled-2-Antibody-Immunohistochemistry-Paraffin-NBP1-58849-img0003.jpg)