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Human VIP ELISA Kit (Colorimetric)

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ELISA: Human VIP ELISA Kit (Colorimetric) [NBP2-82390] - Samples were spiked with high concentrations of Human VIP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Human VIP ELISA Kit (Colorimetric) [NBP2-82390] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL

Order Details

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Human VIP ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human VIP in samples. No Significant cross-reactivity or interference between Human VIP and analogues was observed.
Standard Curve Range
7.81 - 500 pg/mL
Sensitivity
4.69 pg/mL
Assay Type
Competitive-ELISA
Inter-Assay
%CV < 5.05
Intra-Assay
%CV < 4.91
Spike Recovery
86 - 104%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
VIP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human VIP ELISA Kit (Colorimetric)

  • MGC13587
  • PHM27
  • vasoactive intestinal peptide
  • VIP peptides
  • VIP

Background

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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