VIP Antibody (2) - BSA Free

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Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue . Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

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VIP Antibody (2) - BSA Free Summary

Immunogen
Vasoactive intestinal peptide
Specificity
Binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
VIP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:100-1:2000
Application Notes
ELISA: A dilution guideline of 1/1000 applies to VIP coated directly at 2 ug/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 ug/mL, the dilution is 1/32,000. WB: NBP1-05163 can be used in Western blotting. IHC: NBP1-05163 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100. IF: NBP1-05163 was used in IF with a dilution of 1:100.
Publications
Read Publication using
NBP1-05163 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
10mM Phosphate (pH 7.4) and 0.5M NaCl
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for VIP Antibody (2) - BSA Free

  • MGC13587
  • PHM27
  • vasoactive intestinal peptide
  • VIP peptides
  • VIP

Background

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, ICC/IF, IHC

Publications for VIP Antibody (NBP1-05163)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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Product General Protocols

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WB Video Protocol
ICC/IF Video Protocol

FAQs for VIP Antibody (NBP1-05163). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in the NBP1-05163, your anti-VIP mAb. I noticed that the mAb has been tested in a variety of applications, but I'm curious if you are aware of it being used in mice, and more importantly in flow cytometry and IF?
    • In regards to your inquiry unfortunately this antibody has only been tested in ELISA and we have not tested it in Flow cytometry or IF. Due to this antibody being of a mouse host we have not tested on mouse samples due to high background potential. Unfortunately we do not have any samples we can provide, but we do have our Innovator's Reward if you wish to test this in untested species or applications. If you would like to test this antibody in an untested species and/or application and share your results with us, then I can recommend our Innovators Reward Program.
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