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Uroplakin IIIB Antibody (UPK3B/8550R) - Azide and BSA Free

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Formalin-fixed, paraffin-embedded human bladder stained with Uroplakin IIIB antibody (UPK3B/8550R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
Formalin-fixed, paraffin-embedded human bladder stained with Uroplakin IIIB antibody (UPK3B/8550R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA, IHC
Clone
UPK3B/8550R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Uroplakin IIIB Antibody (UPK3B/8550R) - Azide and BSA Free Summary

Description
Positive Controls: Human urinary bladder.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fragment (around aa 100-300) of human Uroplakin IIIB protein (exact sequence is proprietary)
Localization
Cell surface. Heterodimer formation with UPK1B is a prerequisite to exit out of the endoplasmic reticulum.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
UPK3B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Uroplakin IIIB Antibody (UPK3B/8550R) - Azide and BSA Free

  • FLJ32198
  • MGC10902
  • p35
  • UP3b
  • UPIIIB
  • UPIIIbP35
  • uroplakin 3B
  • Uroplakin IIIb
  • uroplakin-3b

Background

UPK3B is a minor component of the apical plaques of mammalian urothelium that binds and dimerizes with uroplakin-1b (UPK1B; MIM 602380), one of the major conserved urothelium membrane proteins. The other major conserved integral membrane proteins of urothelial plaques are UPK1A (MIM 611557), UPK2 (MIM 611558), and UPK3A (MIM 611559) (Deng et al., 2002 [PubMed 12446744]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for Uroplakin IIIB Antibody (NBP3-20914) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UPK3B