![Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis in control (vector only transfected HEK293T lysate) and TWISTNB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/TWISTNB-Antibody-Western-Blot-NBP1-89449-img0009.jpg "Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis in control (vector only transfected HEK293T lysate) and TWISTNB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: QTMEINMGDELEFEVFRLDSDAAGVFCIRGKLNITSLQFKRSEVSEEVTENGTEEAAKKPKKKKKKKDPETYEVDSGTTKLAD |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TWISTNB |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization, Use PFA/Triton X-100. Western Blot and knockdown verified by a customer review. |
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| Control Peptide |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
WB | Human | 09/03/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TWISTNB |
![Immunocytochemistry/Immunofluorescence: TWISTNB Antibody [NBP1-89449] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center.](http://images.novusbio.com/fullsize/TWISTNB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89449-img0008.jpg "Immunocytochemistry/Immunofluorescence: TWISTNB Antibody [NBP1-89449] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center.")
![Immunohistochemistry-Paraffin: TWISTNB Antibody [NBP1-89449] - Staining of human cerebellum shows cytoplasmic and nucleolar positivity in Purkinje cells.](http://images.novusbio.com/fullsize/TWISTNB-Antibody-Immunohistochemistry-Paraffin-NBP1-89449-img0007.jpg "Immunohistochemistry-Paraffin: TWISTNB Antibody [NBP1-89449] - Staining of human cerebellum shows cytoplasmic and nucleolar positivity in Purkinje cells.")
![Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis of 293T cells lysate after siren treatment with siTWISTNB and siControl. Image from verified customer review.](http://images.novusbio.com/fullsize/TWISTNB-Antibody-Western-Blot-NBP1-89449-img0005.jpg "Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis of 293T cells lysate after siren treatment with siTWISTNB and siControl. Image from verified customer review.")
![Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis in human cell line U-937 MG.](http://images.novusbio.com/fullsize/TWISTNB-Antibody-Western-Blot-NBP1-89449-img0006.jpg "Western Blot: TWISTNB Antibody [NBP1-89449] - Analysis in human cell line U-937 MG.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
-(01-ml)_NBP1-89449_9801.jpg)