TSH beta Antibody (1D12G1) - BSA Free

Images

 
Western Blot: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis using TSHB mAb against human TSHB (AA: 20-139) recombinant protein. (Expected MW is 16 kDa)
Immunohistochemistry-Paraffin: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis of paraffin-embedded pancreas tissues using TSHB mouse mAb with DAB staining.
Flow Cytometry: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis of Hela cells using TSHB mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: Thyroid Stimulating Hormone Antibody (1D12G1) [NBP2-52574] - Analysis of paraffin-embedded liver cancer tissues using TSHB mouse mAb with DAB staining.
ELISA: TSH beta Antibody (1D12G1) [NBP2-52574] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
1D12G1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

TSH beta Antibody (1D12G1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human TSH beta (AA: 20-139) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TSHB
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for TSH beta Antibody (1D12G1) - BSA Free

  • thyroid stimulating hormone, beta
  • thyroid-stimulating hormone subunit beta
  • thyrotropin beta chain
  • thyrotropin beta subunit
  • Thyrotropin beta
  • thyrotropin subunit beta
  • TSH beta
  • TSHB
  • TSH-B
  • TSH-BETA

Background

Thyroid-stimulating hormone (TSH), also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs, finds its receptors on epithelial cells in the thyroid gland, and stimulates that gland to synthesize and release thyroid hormones. TSH is a glycoprotein hormone composed of two subunits which are non-covalently bound to one another. The alpha subunit of TSH is also present in two other pituitary glycoprotein hormones, follicle-stimulating hormone and luteinizing hormone, and, in primates, in the placental hormone chorionic gonadotropin. Each of these hormones also has a unique beta subunit, which provides receptor specificity. In other words, TSH is composed of alpha subunit bound to the TSH beta subunit, and TSH associates only with its own receptor. Free alpha and beta subunits have essentially no biological activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TSHB