![Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Western-Blot-NB100-56748-img0002.jpg "Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml."){kind=icon}
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 12A380 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TNFSF11 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
![Western Blot PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Western_Blot_16656.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for TRANCE/TNFSF11/RANK L Antibody (NB100-56748)Find related products by research area.
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The diverse functions of RANKL/TRANCE/TNFSF11 RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t... Read full blog post. |
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![Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Immunohistochemistry-Paraffin-NB100-56748-img0010.jpg "Immunohistochemistry-Paraffin: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz."){kind=icon}
![Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Flow-Cytometry-NB100-56748-img0006.jpg "Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay."){kind=icon}
![Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody. Image using the PE format of this antibody.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Immunohistochemistry-Frozen-NB100-56748-img0011.jpg "Immunohistochemistry-Frozen: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis in RAW cells using 2 ug of antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody. Image using the PE format of this antibody."){kind=icon}
![Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.](http://images.novusbio.com/fullsize/TRANCE-TNFSF11-RANK-L-Antibody-12A380-Flow-Cytometry-NB100-56748-img0005.jpg "Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) [NB100-56748] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56748. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay."){kind=icon}
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")