Home » TRA-1-85/CD147 » TRA-1-85/CD147 Antibodies » TRA-1-85/CD147 Antibody (TRA-1-85) [Unconjugated]
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TRA-1-85/CD147 Antibody (TRA-1-85) [Unconjugated]

 (14 Publications)
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Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # ...read more
Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA‑1‑85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by ...read more
TRA-1-85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRA-1-85/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF, KO
Clone
TRA-1-85
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3195U-100UG
Alexa Fluor 405
FAB3195V-100UG
Alexa Fluor 488
FAB3195G-100UG
Alexa Fluor 532
FAB3195X
Alexa Fluor 594
FAB3195T-100UG
Alexa Fluor 647
FAB3195R-100UG
Alexa Fluor 700
FAB3195N
Alexa Fluor 750
FAB3195S-100UG
Allophycocyanin
FAB3195A
Allophycocyanin/Cy7
FAB3195APCCY7
Biotin
BAM3195
CoraFluor 1
FAB3195CL1
DyLight 350
FAB3195D
DyLight 405
FAB3195E
DyLight 488
FAB3195K
DyLight 550
FAB3195L
DyLight 594
FAB3195M
DyLight 650
FAB3195W
DyLight 680
FAB3195Y
DyLight 755
FAB3195Z
Fluorescein
FAB3195F
HRP
FAB3195H
Janelia Fluor 525
FAB3195JF525
Janelia Fluor 549
FAB3195I
Janelia Fluor 585
FAB3195JF585
Janelia Fluor 635
FAB3195JF635
Janelia Fluor 646
FAB3195J
Janelia Fluor 669
FAB3195JF669
PE/Atto594
FAB3195PEATT594
PE/Cy5.5
FAB3195PECY55
PE/Cy7
FAB3195PECY7
Phycoerythrin
FAB3195P
mFluor Violet 450 SE
FAB3195MFV450
mFluor Violet 500 SE
FAB3195MFV500
mFluor Violet 610 SE
FAB3195MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB3195F
Carrier Free
FAB3195P
Carrier Free
FAB3195A
Carrier Free
FAB3195N
Carrier Free
MAB3195-SP
Carrier Free
FAB3195V-100UG
Carrier Free
FAB3195U-100UG
Carrier Free
FAB3195T-100UG
Carrier Free
FAB3195S-100UG
Carrier Free
FAB3195R-100UG
Carrier Free
FAB3195G-100UG
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TRA-1-85/CD147 Antibody (TRA-1-85) [Unconjugated] Summary

Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Detects human TRA‑1‑85 antigen in Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BSG
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB3195 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRA-1-85/CD147 Antibody (TRA-1-85) [Unconjugated]

  • OKa Blood Group Antigen
  • TRA185
  • TRA-1-85

Background

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).
  1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.
  2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-85/CD147 Antibody (MAB3195)(14)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rats.

We have publications tested in 5 applications: Functional Assay, ICC, IHC, IHC-Fr, Neutralization.


Filter By Application
Functional Assay
(1)
ICC
(2)
IHC
(5)
IHC-Fr
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Rat
(2)
Bovine
(1)
Rats
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB3195 Applications Species
Jamwal, A;Constantin, CF;Hirschi, S;Henrich, S;Bildl, W;Fakler, B;Draper, SJ;Schulte, U;Higgins, MK; Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes eLife 2023-10-05 [PMID: 37796723]
Nagai, K;Muto, Y;Miura, S;Takahashi, K;Naruse, Y;Hiruta, R;Hashimoto, Y;Uzuki, M;Haga, Y;Fujii, R;Ueda, K;Kawaguchi, Y;Fujii, M;Kitazume, S; Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth PTPRZ glycosylation regulates glioma growth The Journal of biological chemistry 2023-08-03 [PMID: 37543361] (IHC, Mouse, Human) IHC Mouse, Human
Titilola D. Kalejaiye, Rohan Bhattacharya, Morgan A. Burt, Tatianna Travieso, Arinze E. Okafor, Xingrui Mou et al. SARS-CoV-2 Employ BSG/CD147 and ACE2 Receptors to Directly Infect Human Induced Pluripotent Stem Cell-Derived Kidney Podocytes Frontiers in Cell and Developmental Biology 4/20/2022 [PMID: 35517495]
DS Rajendran, D Zhu, R Sharma, JC Martinez C, K Bharti, DR Hinton, MS Humayun, BB Thomas Long-Term Transplant Effects of iPSC-RPE Monolayer in Immunodeficient RCS Rats Cells, 2021-10-29;10(11):. 2021-10-29 [PMID: 34831174] (IHC, Rats) IHC Rats
T Nakamura, U Yokoyama, T Kanaya, T Ueno, T Yoda, A Ishibe, Y Hidaka, M Umemura, T Takayama, M Kaneko, S Miyagawa, Y Sawa, I Endo, Y Ishikawa Multilayered Human Skeletal Muscle Myoblast Sheets Promote the Healing Process After Colonic Anastomosis in Rats Cell Transplantation, 2021-01-01;30(0):9636897211009. 2021-01-01 [PMID: 33880968] (IHC, Rat) IHC Rat
BO Pennington, JK Bailey, MA Faynus, C Hinman, MN Hee, R Ritts, V Nadar, D Zhu, D Mitra, JC Martinez-C, TC Lin, BB Thomas, DR Hinton, MS Humayun, J Lebkowski, LV Johnson, DO Clegg Xeno-free cryopreservation of adherent retinal pigmented epithelium yields viable and functional cells in vitro and in vivo Scientific Reports, 2021-03-18;11(1):6286. 2021-03-18 [PMID: 33737600] (ICC, IHC, Bovine, Human, Rat) ICC, IHC Bovine, Human, Rat
Thomas C. Chen, Nymph Chan, Radu O. Minea, Hannah Hartman, Florence M. Hofman, Axel H. Schönthal Rare Stochastic Expression of O6-Methylguanine- DNA Methyltransferase (MGMT) in MGMT-Negative Melanoma Cells Determines Immediate Emergence of Drug-Resistant Populations upon Treatment with Temozolomide In Vitro and In Vivo Cancers (Basel) 9/28/2018 [PMID: 30274152]
R Xu, J Ji, X Zhang, M Han, C Zhang, Y Xu, Y Wei, S Wang, B Huang, A Chen, D Zhang, Q Zhang, W Li, Z Jiang, J Wang, X Li PDGFA/PDGFR?-regulated GOLM1 promotes human glioma progression through activation of AKT J. Exp. Clin. Cancer Res., 2017-12-28;36(1):193. 2017-12-28 [PMID: 29282077] (ICC, Human) ICC Human
Ronald A DePinho Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth Cell, 2016-11-17;167(5):1281-1295.e18. 2016-11-17 [PMID: 27863244] (IHC, Human) IHC Human
Michael J. Koss, Paulo Falabella, Francisco R. Stefanini, Marcel Pfister, Biju B. Thomas, Amir H. Kashani et al. Subretinal implantation of a monolayer of human embryonic stem cell-derived retinal pigment epithelium: a feasibility and safety study in Yucatán minipigs Graefe's Archive for Clinical and Experimental Ophthalmology 8/1/2016 [PMID: 27335025]
Show All 14 Publications.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BSG