Human TNF RII/TNFRSF1B ELISA Kit (Colorimetric)

Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNF alpha, also called cachectin, and TNF beta, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects. Both TNF alpha and TNF beta have been shown to interact with a cell through specific high affinity receptors with a few hundred up to more than 20,000 copies per cell. TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Normal and malignant human myeloid cells as well as mitogen- stimulated lymphocytes express similar numbers of TNF receptors (400-1,900 per cell), whereas resting lymphoid cells have fewer, red blood cells and platelets have no detectable TNF receptors. In most cases no correlation is observed between receptor number and sensitivity to TNF. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75 - 80 and 55 - 60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The predicted amino acid sequences of the extracellular regions of the two TNF-R reveal significant similarities. The 60 kDa receptor consists of 426 amino acids with a single membrane span, an extracellular domain of 182 amino acids and an intracellular domain of 223 amino acids. The 80 kDa receptor is also a single membrane-spanning receptor of 439 amino acids with an extracellular domain of 235 amino acids and an intracellular domain of 178 amino acids. They share 28 % identity on their extracellular domain, but their intracellular parts are totally different from each other

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Array NBP1-88105

• TNF RII/TNFRSF1B Antibodies
• TNF RII/TNFRSF1B Antibody Pair
• TNF RII/TNFRSF1B ELISA Kits
• TNF RII/TNFRSF1B Lysate
• TNF RII/TNFRSF1B Proteins
• TNF RII/TNFRSF1B ELISAs
• TNF RII/TNFRSF1B Proteins and Enzymes