Tat-Beclin 1 L11 Autophagy Inducing Peptide

Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Many standard methods to induce autophagy lack desired specificity. Both starvation and rapamycin, an allosteric inhibitor of MTORC1, are known to regulate biological processes other than autophagy. The non-specific nature of these methods complicates data interpretation and has driven the preference for loss-of-function Atg mutants to analyze autophagy. In 2013, the discovery of engineered Tat-Beclin 1 provided the first method to induce autophagy without regulating other pathways non-specifically. Peptides composed of the autophagy-inducing region of Beclin 1 fused to the HIV-Tat protein were demonstrated to increase autophagosome and autolysosome numbers, as well as protein degradation. Since then, Tat-Beclin 1 peptides have been widely used to successfully induce autophagy both in vitro and in vivo. The peptides below are of a shorter Tat-Beclin 1 peptide with an enhanced potency to induce autophagy. This shorter peptide, Tat-D11, increases autophagosome and autolysosome induction by over fivefold compared to the longer peptide, Tat-Beclin 1. Results have demonstrated the superior potency of Tat-D11 over Tat-Beclin 1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: In Vivo, In vivo assay.

Showing Publications 1 - 7 of 7.

Publications using NBP2-49886	Applications	Species
Zagkou S, Marais V, Zeghoudi N et al. Design and Evaluation of Autophagy-Inducing Particles for the Treatment of Abnormal Lipid Accumulation Pharmaceutics 2022-06-29 [PMID: 35890275] (In vivo assay, Mouse)	In vivo assay	Mouse
Vega-Rubin-de-Celis S, Zou Z, Fernandez AF et al. Increased autophagy blocks HER2-mediated breast tumorigenesis Proc Natl Acad Sci U S A 2019-04-17 [PMID: 29610308] (In Vivo, Human)	In Vivo	Human
Bartolomeo R, Cinque L, De Leonibus C mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017-10-02 [PMID: 28872463] (In Vivo, Mouse)	In Vivo	Mouse
Peraro L, Zou Z, Makwana KM et al. Diversity-Oriented Stapling Yields Intrinsically Cell-Penetrant Inducers of Autophagy J Am Chem Soc. 2017-06-14 [PMID: 28414223] (In Vivo, Human)	In Vivo	Human
Franco LH, Nair VR, Scharn CR et al. The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense. Cell Host Microbe 2017-01-11 [PMID: 28017659] (In Vivo, Mouse)	In Vivo	Mouse
Pietrocola F, Pol J Vacchelli E et al. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance Cancer Cell. 2016-07-11 [PMID: 27411589] (In Vivo, Mouse)	In Vivo	Mouse
Shoji-Kawata S, Sumpter R, Leveno M et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013-02-14 [PMID: 23364696] (In Vivo) Details: Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4).	In Vivo

Array NBP2-49886

• Tat-Beclin 1 Proteins