Tat-Beclin 1 D11 Autophagy Inducing Peptide - Retroinverso form

Note: Tat-L11 and Tat-D11, are exclusively available from Novus Biologicals (Bio-Techne's exclusive patent license: US Patent 8,802,633).

Many standard methods to induce autophagy lack desired specificity. Both starvation and rapamycin, an allosteric inhibitor of MTORC1, are known to regulate biological processes other than autophagy. The non-specific nature of these methods complicates data interpretation and has driven the preference for loss-of-function Atg mutants to analyze autophagy. In 2013, the discovery of engineered Tat-Beclin 1 provided the first method to induce autophagy without regulating other pathways non-specifically. Peptides composed of the autophagy-inducing region of Beclin 1 fused to the HIV-Tat protein were demonstrated to increase autophagosome and autolysosome numbers, as well as protein degradation. Since then, Tat-Beclin 1 peptides have been widely used to successfully induce autophagy both in vitro and in vivo. The peptides below are of a shorter Tat-Beclin 1 peptide with an enhanced potency to induce autophagy. This shorter peptide, Tat-D11, increases autophagosome and autolysosome induction by over fivefold compared to the longer peptide, Tat-Beclin 1. Results have demonstrated the superior potency of Tat-D11 over Tat-Beclin 1.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Func, In Vivo, In vitro.

Showing Publications 1 - 10 of 21. Show All 21 Publications. Collapse Publications.

Publications using NBP2-49888	Applications	Species
Khang Nguyen, Jialing Tang, Sungji Cho, Fan Ying, Hye Kyoung Sung, James Wonsuk Jahng, Kostas Pantopoulos, Gary Sweeney Salubrinal promotes phospho-eIF2 alpha -dependent activation of UPR leading to autophagy-mediated attenuation of iron-induced insulin resistance Molecular Metabolism 2024-03-26 [PMID: 38527647]
Atsushi F, Tomoko S, Mihoko Y et al. Chloroquine is a safe autophagy inhibitor for sustaining the expression of antioxidant enzymes in trophoblasts Journal of Reproductive Immunology 2022-11-01 [PMID: 36470134]
Zheng N, Fang J, Xue G et al. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance Cancer cell 2022-08-15 [PMID: 36027915]
Luis LB, Ana GT, Carlos GE et al. Salmonella Promotes Its Own Survival in B Cells by Inhibiting Autophagy Cells 2022-06-29 [PMID: 35805144] (In vitro)	In vitro
Forte M, Bianchi F, Cotugno M Et al. An interplay between UCP2 and ROS protects cells from high-salt-induced injury through autophagy stimulation Cell death & disease 2021-10-08 [PMID: 34625529] (In vitro)	In vitro
Pavel M, Park SJ, Frake RA et al. alpha-Catenin levels determine direction of YAP/TAZ response to autophagy perturbation Nature communications 2021-03-17 [PMID: 33731717]
Ramesh B. Kasetti, Prabhavathi Maddineni, Charles Kiehlbauch, Shruti Patil, Charles C. Searby, Beth Levine, Val C. Sheffield, Gulab S. Zode Autophagy stimulation reduces ocular hypertension in a murine glaucoma model via autophagic degradation of mutant myocilin JCI Insight 2021-03-08 [PMID: 33539326]
Yang J, Kitami M, Pan H, et al. Augmented BMP signaling commits cranial neural crest cells to a chondrogenic fate by suppressing autophagic beta-catenin degradation Science signaling 2021-01-12 [PMID: 33436499]
Soria L, Perocheau D, De Sabbata G et al. Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders EMBO Mol Med 2020-12-28 [PMID: 33369168]
Mathew B, Chennakesavalu M, Sharma M et al. Autophagy and post-ischemic conditioning in retinal ischemia Autophagy 2020-05-26 [PMID: 32452260] (In vitro, Rat)	In vitro	Rat
Iaconis D, Crina C, Brillante S et al. The HOPS Complex Subunit VPS39 controls ciliogenesis through autophagy Hum. Mol. Genet. 2020-02-20 [PMID: 32077937] (Human)		Human
Forte M, Bianchi F, Cotugno M et al. Pharmacological restoration of autophagy reduces hypertension-related stroke occurrence Autophagy. 2019-11-12 [PMID: 31679456]
Watanabe R, Sasaki S, Kimura N Activation of autophagy in early neonatal mice increases primordial follicle number and improves lifelong fertility Biol. Reprod. 2019-09-30 [PMID: 31566206]
Wong M, Ganapathy AS, Suchanec E et al. Intestinal Epithelial Tight Junction Barrier regulation by Autophagy related protein ATG6/beclin 1 Am. J. Physiol., Cell Physiol. 2019-03-20 [PMID: 30892937] (Func)	Func
Sharif T, Martell E, Dai C et al. HDAC6 differentially regulates autophagy in stem-like versus differentiated cancer cells. Autophagy. 2018-11-16 [PMID: 30444165]
Vega-Rubin-de-Celis S, Zou Z, Fernandez AF et al. Increased autophagy blocks HER2-mediated breast tumorigenesis Proc Natl Acad Sci U S A 2019-04-17 [PMID: 29610308] (In Vivo, Human)	In Vivo	Human
Bartolomeo R, Cinque L, De Leonibus C mTORC1 hyperactivation arrests bone growth in lysosomal storage disorders by suppressing autophagy. J Clin Invest. 2017-10-02 [PMID: 28872463] (In Vivo, Mouse)	In Vivo	Mouse
Peraro L, Zou Z, Makwana KM et al. Diversity-Oriented Stapling Yields Intrinsically Cell-Penetrant Inducers of Autophagy J Am Chem Soc. 2017-06-14 [PMID: 28414223] (In Vivo, Human)	In Vivo	Human
Franco LH, Nair VR, Scharn CR et al. The Ubiquitin Ligase Smurf1 Functions in Selective Autophagy of Mycobacterium tuberculosis and Anti-tuberculous Host Defense. Cell Host Microbe 2017-01-11 [PMID: 28017659] (In Vivo, Mouse)	In Vivo	Mouse
Pietrocola F, Pol J Vacchelli E et al. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance Cancer Cell. 2016-07-11 [PMID: 27411589] (In Vivo, Mouse)	In Vivo	Mouse
Shoji-Kawata S, Sumpter R, Leveno M et al. Identification of a candidate therapeutic autophagy-inducing peptide. Nature. 2013-02-14 [PMID: 23364696] (In Vivo) Details: Tat-beclin 1 (L-amino acid) / Tat-Beclin 1 L11 (NBP2-49886) and Tat–beclin 1 (D-amino acid)/Tat-Beclin 1 D11, Retroinverso form (NBP2-49888) along with the control peptide Tat-scrambled (L-amino acid)/Tat-Beclin 1 L11S Peptide, Scrambled Control (NBP2-49887) were tested in-vivo for the induction of autophagy. 6-week-old GFP-LC3 transgenic mice, and normal or CHIKV and WNV Egypt strain virus infected 5-day-old GFP-LC3 mice were injected intra-peritoneally (i.p.) with these Tat-beclin derivative peptides at a dose of 20 mg/Kg body weight (5.3uM/Kg). Brain tissues were analyzed using IHC-Frozen and Western blot analysis for measuring cell death (TUNEL Assay) and p62 expression respectively. Toxicity of these peptides was assessed in 6-day-old C57BL/6J mice via daily injections of Tat-scrambled (L-amino acid, Tat-Beclin 1 L11S) or Tat-beclin 1 (L-amino acid, Tat-Beclin 1 L11) at 15 mg kg-1 and Tat–beclin 1 (D-amino acid, Tat-Beclin 1 D11) at a dose of 20 mg/Kg body weight for 2 weeks (Figure 4).	In Vivo
Show All 21 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	In vivo treatment + immunoblot analysis		01/07/2019	View Summary Application Other Lot 1455072 Comments Increased basal expression of LC3-II on immunoblot as expected in the kidneys at a dose of 1mg/kg.Soluble in PBS. Comments Comments I used this peptide for an in vivo dose study in 10wk old C57BL/6J mice. I administered either 1mg/kg or 10mg/kg IP once daily for 2 days, sacrificed the mice, and prepared the kidneys for western blot analysis (image shown). Additionally, the lysosomal inhibitor bafilomycin A1 was used to provide a measurement of autophagic flux. *vehicle is scrambled tat-beclin (NBP2-49887-5mg)

Array NBP2-49888

• Tat-Beclin 1 Proteins