![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (3B1NB) conjugated to Alexa Fluor 488 [NBP2-44310AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44311-img0005.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (3B1NB) conjugated to Alexa Fluor 488 [NBP2-44310AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | 3B1NB |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide Free |
| Immunogen | Partial recombinant human Spectrin beta 3 protein (between amino acids 1300-1500) [UniProt O15020] |
| Localization | Cytoplasm, Cytoskeleton, Cell junctions |
| Predicted Species | Rat (96%). Backed by our 100% Guarantee. |
| Isotype | IgG3 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPTBN2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SPTBN2 |
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human prostate using 1:200 dilution of Spectrin beta 3 antibody (clone 3B1NB). The antibody generated a specific cytoplasmic staining with strong signal at cell-cell junctions in the glandular epithelium of the prostate section. The cells of fibromuscular stroma showed a diffused signal throughout the sections. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44311-img0006.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human prostate using 1:200 dilution of Spectrin beta 3 antibody (clone 3B1NB). The antibody generated a specific cytoplasmic staining with strong signal at cell-cell junctions in the glandular epithelium of the prostate section. The cells of fibromuscular stroma showed a diffused signal throughout the sections. Image using the standard format of this product.")
![Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - Spectrin B3 antibody (clone 3B1NB) was used to stain HeLa cells (1 x 10^6 cells/ml) at a 1:500 dilution detected using Dylight-488 conjugated goat anti-rat IgG secondary antibody (blue peak). Shown with secondary control (orange peak).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Flow-Cell-Surface-NBP2-44311-img0003.jpg "Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - Spectrin B3 antibody (clone 3B1NB) was used to stain HeLa cells (1 x 10^6 cells/ml) at a 1:500 dilution detected using Dylight-488 conjugated goat anti-rat IgG secondary antibody (blue peak). Shown with secondary control (orange peak).")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - A431 cells were fixed for 10 minutes using -20C MeOH. The cells were incubated with anti-Spectrin beta 3 (3B1NB) [NBP2-44311-azide free] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44311-img0004.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - A431 cells were fixed for 10 minutes using -20C MeOH. The cells were incubated with anti-Spectrin beta 3 (3B1NB) [NBP2-44311-azide free] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
