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Siglec-1/CD169 Antibody (7-239) - BSA Free

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Flow Cytometry: Siglec-1/CD169 Antibody (7-239) [NBP2-37729] - Staining of CD169 on buffy coat diff. monocytes by anti-CD169 (7-239) PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
7-239
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Siglec-1/CD169 Antibody (7-239) - BSA Free Summary

Immunogen
Human rhinovirus 14-infected monocyte-derived dendritic cells.
Specificity
The mouse monoclonal antibody 7-239 recognizes CD169 (sialoadhesin, Siglec-1), a 210 kDa type I transmembrane glycoprotein expressed on macrophages and dendritic cells.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIGLEC1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Blocking/Neutralizing: inhibition of erythrocyte rosetting with cells expressing cd169. This antibody is CyTOF ready.
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-37729 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Siglec-1/CD169 Antibody (7-239) - BSA Free

  • CD169
  • FLJ00051
  • sialic acid binding Ig-like lectin 1, sialoadhesin
  • sialoadhesin
  • Siglec1
  • Siglec-1

Background

The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions. Family members include sialoadhesin, CD22 and CD33. Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Siglec-1/CD169 Antibody (NBP2-37729) (0)

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Review for Siglec-1/CD169 Antibody (NBP2-37729) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-37729:
Filter by Applications
ICC
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Siglec-1/CD169 NBP2-37729
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5
reviewed by:
Jason Hammonds
ICC Human 03/23/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary human macrophages
SpeciesHuman
LotN/A

Comments

CommentsIF staining of Siglec-1 in GMCSF matured, primary human macrophages at 1:200. DAKO blocking and background reducing antibody diluent. DAPI nuclear stain.

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
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1
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Jason Hammonds
03/23/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SIGLEC1