Western Blot: Sars Membrane Protein Antibody [NB100-56569] - Western blot analysis of Sars Membrane Protein in (A) recombinant fusion protein containing amino acids 195-210 and (B) fusion partner without these amino ...read more
Immunocytochemistry/ Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569] - Vero cells infected with SARS-CoV-2. Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid ...read more
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 195-210 (YSRYRIGNYKLNTDH) from the M (Membrane protein) for the Human SARS coronavirus (Genbank accession no. YP_009724393.1)
Specificity
The NCBI accession number of the SARS matrix protein is NP_828855.1.
Predicted Species
SARS-CoV-2 (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunogen displays the following percentage of sequence identity for non-tested species: COVID-19 (93%)
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified
Alternate Names for Sars Membrane Protein Antibody
E1 glycoprotein
Matrix glycoprotein
Membrane glycoprotein
SARS coronavirus M protein
SARS coronavirus Membrane protein
SARS CoV M glycoprotein
SARS CoV Matrix protein
SARS CoV
Sars M
Sars Matrix Protein
SARS-CoV M protein
SARS-CoV Membrane protein
SARSCoV
Severe acute respiratory syndrome
Background
A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The M protein (Membrane protein, Matrix protein) is one of the major structural viral proteins. It is an integral membrane protein involved in the budding of the viral particles and interacts with S (Spike) protein and the nucleocapsid protein. Coronaviruses have four important viral genes with different structural proteins: a spike glycoprotein (S), a small envelope protein (E), a matrix glycoprotein (M), and a nucleocapsid protein (N).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Sars Membrane Protein Antibody (NB100-56569)(4)
Review for Sars Membrane Protein Antibody (NB100-56569) (1)
51
Average Rating: 5 (Based on 1 review)
We have 1 review tested in 1 species: Monkey.
Reviews using NB100-56569:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Monkey
(1)
All Species
Images
Ratings
Applications
Species
Date
Details
5
reviewed by: Luca Mascheroni
ICC
Monkey
02/04/2022
View
Summary
Application
Immunocytochemistry
Sample Tested
Vero cell
Species
Monkey
Lot
IN021405D-4
Comments
Comments
***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Sars Membrane Protein Antibody (NB100-56569). (Showing 1 - 1 of 1 FAQs).
Has it been tested whether the two antobodies anti-M protein from SARS-CoV cross-react with that of SARS-CoV-2? The references are 56569SS and NBP1-28852.
Sars Membrane Protein Antibody NB100-56569 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 93%. Sars Membrane Protein Antibody (2H2C4)NBP1-28852 expected cross reactivity with SARS-CoV-2 based on immunogen sequence homology: 88%
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Sars Membrane Protein Antibody [NB100-56569] - Western blot analysis of Sars Membrane Protein in (A) recombinant fusion protein containing amino acids 195-210 and (B) fusion partner without these amino acids, using NB100-56569 at 0.5 ug/ml.](http://images.novusbio.com/fullsize/Sars-Membrane-Protein-Antibody-Western-Blot-NB100-56569-img0003.jpg "Western Blot: Sars Membrane Protein Antibody [NB100-56569] - Western blot analysis of Sars Membrane Protein in (A) recombinant fusion protein containing amino acids 195-210 and (B) fusion partner without these amino acids, using NB100-56569 at 0.5 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569] - Vero cells infected with SARS-CoV-2. Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid protein. Image from verified customer review.](http://images.novusbio.com/fullsize/Sars-Membrane-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56569-img0004.jpg "Immunocytochemistry/Immunofluorescence: Sars Membrane Protein Antibody [NB100-56569] - Vero cells infected with SARS-CoV-2. Blue: DAPI; Green: SARS-CoV-2 membrane protein (NB100-56569); Magenta: SARS-CoV-2 nuclecapsid protein. Image from verified customer review.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
