Human Ryanodine Receptor 1 ELISA Kit (Colorimetric) Summary
Description |
Assay Length: 4.5 hours |
Standard Curve Range |
0.781 - 50 ng/mL (example only; lot dependent) |
Sensitivity |
0.27 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 12 (example only; lot dependent) |
Intra-Assay |
%CV < 10 (example only; lot dependent) |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
RYR1 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
|
Alternate Names for Human Ryanodine Receptor 1 ELISA Kit (Colorimetric)
Background
The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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