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Human Ryanodine Receptor 1 ELISA Kit (Colorimetric)

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ELISA: Human Ryanodine Receptor 1 ELISA Kit (Colorimetric) [NBP3-40406] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.781 - 50 ng/mL (example only; lot dependent)
Sensitivity
0.27 ng/mL (example only; lot dependent)

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Human Ryanodine Receptor 1 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
0.781 - 50 ng/mL (example only; lot dependent)
Sensitivity
0.27 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
RYR1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Ryanodine Receptor 1 ELISA Kit (Colorimetric)

  • CCO
  • central core disease of muscle
  • MHS
  • MHS1
  • PPP1R137
  • protein phosphatase 1, regulatory subunit 137
  • ryanodine receptor 1 (skeletal)
  • Ryanodine Receptor 1
  • ryanodine receptor type1
  • RYDR
  • RYR
  • RyR1
  • RYR-1
  • sarcoplasmic reticulum calcium release channel
  • Skeletal muscle calcium release channel
  • skeletal muscle ryanodine receptor
  • Skeletal muscle-type ryanodine receptor
  • SKRR
  • type 1-like ryanodine receptor

Background

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol RYR1