Rituximab Antibody (2260A) [Unconjugated]

Images

 
Western blot shows Cetuximab (negative control), Rituximab, Brentuximab (negative control), and Gemtuzumab (negative control) biosimilars. PVDF membrane was probed with 0.5 µg/mL of Anti-Rituximab (Anti-Idiotype) ...read more
In a functional flow cytometry test, Biotinylated Anti-MS4A1/CD20 (Rituximab) (Catalog # FAB9575B) binds to HEK293 human embryonic kidney cell line transfected with human MS4A1/CD20 (black dotted line). Binding is ...read more
HEK293 human cell line transfected with (A) human MS4A1/CD20 or (B) irrelevant transfectants and eGFP were stained with Human anti-Human MS4A1/CD20 (Rituximab) Monoclonal Antibody (MAB9575), then Anti-Rituximab ...read more
Biotinylated biosimilars were serially diluted and incubated with Anti-Rituximab (Anti-Idiotype) Antibody (Catalog # MAB9630, green line) coated at 1 μg/mL on a 96-well polystyrene plate. No cross-reactivity with ...read more
A panel of biosimilars was coated at 1 μg/mL in 96-well polystyrene plates. Anti-Rituximab (Anti-Idiotype) Antibody (Catalog # MAB9630, 333 ng/mL) detected Rituximab. 100% cross-reactivity with Tositumomab and ...read more
The anti-idiotype antibody (catalog number MAB9630) directed against Rituximab was coated at 4 μg/mL in a 96-well polystyrene plate to capture the biosimilar Rituximab. Biotinylated anti-Rituximab (Anti-Idiotype) ...read more
200x105 human NK cells were seeded from PBMCs and cultured for 13 days in plates/flasks using plate-bound Anti-Human NKp46 Monoclonal Antibody in ExCellerate Human NK Cell Expansion Media (Xeno-Free) plus cytokines (see ...read more

Product Details

Summary
Reactivity All-MultiSpecies Glossary
Applications WB, ELISA, Flow, B/N
Clone
2260A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Rituximab Antibody (2260A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Full-length Rituximab
Specificity
Detects Rituximab in Western blots in direct ELISAs. 100% cross-reactivity with Tositumomab and Ibritumomab (95% or higher sequence identity with Rituximab) and no cross-reactivity with Cetuximab, Trastuzumab, Brentuximab, Gemtuzumab, Adalimumab, Solanezumab, Teprotumumab, and Aducanumab is observed.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Binding Inhibition 2.5 ug/mL
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rituximab Antibody (2260A) [Unconjugated]

  • B lymphocyte antigen CD20
  • B lymphocyte surface antigen B1
  • Bp35
  • CD20
  • LEU 16
  • LEU16
  • Membrane spanning 4 domains subfamily A member 1
  • MS4A1
  • Rituximab

Background

The anti-Rituximab antibody is an anti-Idiotype antibody raised against and selected for the antigenic determinants of the variable region of Rituximab. The antibody has been tested against a broad range of other biosimilars to ensure specificity. This highly specialized anti-idiotypic antibody can be used in a variety of pharmacokinetic (PK) assay formats. In addition to Rituximab, the anti-Idiotype antibody also detects derivatives of Rituximab (Ibritumumab and Tositumumab).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rituximab Antibody (MAB9630) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Rituximab Antibody (MAB9630) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Rituximab Products

Array MAB9630

Blogs on Rituximab.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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