Recombinant E. coli Ribonuclease Inhibitor His Protein Summary
Description |
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-155 of E.coli Ribonuclease Inhibitor Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLKQVEI FTDGSCLGNP GPGGYGAILR YRGREKTFSA GYTRTTNNRM ELMAAIVALE ALKEHCEVIL STDSQYVRQG ITQWIHNWKK RGWKTADKKP VKNVDLWQRL DAALGQHQIK WEWVKGHAGH PENERCDELA RAAAMNPTLE DTGYQVEV |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein |
Gene |
RNH1 |
Purity |
>95%, by SDS-PAGE |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2 mM DTT |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
>95%, by SDS-PAGE |
Alternate Names for Recombinant E. coli Ribonuclease Inhibitor His Protein
Background
rnhA is an endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Localized to the nucleus, this protein mediates the removal of Okazaki fragment RNA primers that are present on the lagging strand during DNA replication. rnhA catalyzes the endonucleolytic cleavage of RNA to a 5'-phosphomonoester and is able to bind magnesium or manganese as cofactors. Recombinant e.coli rnhA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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