| Reactivity | MuSpecies Glossary |
| Applications | Bioactivity |
| Clonality | Polyclonal |
| Host | Mouse |
| Format | Carrier-Free |
| Concentration | LYOPH |
| Description | This product is provided in a carrier free format and does NOT contain BSA. |
| Details of Functionality | Bioassay data are not available. |
| Source | Mouse myeloma cell line, NS0-derived mouse IgG2A protein Glu98-Lys330, with an N-terminal Met-Asp-Pro |
| Accession # | |
| N-terminal Sequence | Met |
| Structure / Form | Disulfide-linked homodimer |
| Protein/Peptide Type | Recombinant Proteins |
| Isotype | IgG2a |
| Clonality | Polyclonal |
| Host | Mouse |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Endotoxin Note | <0.01 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 26.7 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| SDS-PAGE | 34-35 kDa, reducing conditions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Reconstitution Instructions | It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL. |
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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