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Recombinant Mouse IFN-kappa Protein, CF

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Recombinant Mouse IFN-kappa (Catalog # 8437-MK/CF) exhibits anti-viral activity in L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.
Recombinant Mouse IFN-kappa (Catalog # 8437-MK/CF) exhibits anti-viral activity in L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
8437-MK-010
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Recombinant Mouse IFN-kappa Protein, CF Summary

Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.2-1.2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IFN-kappa protein
Leu22-Lys199
Accession #
N-terminal Sequence
Leu22
Protein/Peptide Type
Recombinant Proteins
Gene
IFNK
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19-22 kDa, reducing conditions
Publications
Read Publication using
8437-MK/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-kappa Protein, CF

  • IFNK
  • IFNkappa
  • IFN-kappa

Background

Interferon (IFN)-kappa is a member of the type I IFN family, which also includes IFN-alpha , -beta , -epsilon , and -omega . Mouse IFN-kappa is expressed at low levels in peritoneal macrophages and its expression is up-regulated by double-stranded (ds) RNA and IFN-gamma (1). Mice over-expressing IFN-kappa in pancreatic beta  cells developed type I diabetes, similar to what has been reported for mice over-expressing IFN-alpha , -beta , and -gamma (1-4). Mouse IFN-kappa shares 68% and 30% amino acid sequence identity with rat and human IFN-kappa , respectively. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity (5-7). Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression (8-11).
  1. Vassileva, G. et al. (2003) J. Immunol. 170:5748.
  2. Stewart, T.A. et al. (1993) Science 260:1942.
  3. Sarvetnick, N. et al. (1988) Cell 52:773.
  4. Pelegrin, M. et al. (1998) J. Biol. Chem. 273:12332.
  5. LaFleur, D.W. et al. (2001) J. Biol. Chem. 276:39765.
  6. Nardelli, B. et al. (2002) J. Immunol. 169:4822.
  7. Buontempo, P.J. et al. (2006) J. Interferon Cytokine Res. 26:40.
  8. Rincon-Orozco, B. et al. (2009) Cancer Res. 69:8718.
  9. DeCarlo, C.A. et al. (2010) Lab. Invest. 90:1482.
  10. Reiser, J. et al. (2011) J. Virol. 85:11372.
  11. Sunthamala, N. et al. (2014) PLoS One 9:e91473.

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Publications for IFN-kappa (8437-MK/CF)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol IFNK
Uniprot