Home » FGF acidic/FGF1 » FGF acidic/FGF1 Proteins » Recombinant Mouse FGF acidic/FGF1 Protein, CF

Recombinant Mouse FGF acidic/FGF1 Protein, CF

 (8 Publications)
 See All Related
Submit a Review

Images

 
There are currently no images for FGF acidic/FGF1 (4686-FA/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
4686-FA-025
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Mouse FGF acidic/FGF1 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED50 for this effect is 0.05-0.25 ng/mL in the presence of 10 µg/mL heparin.
Source
E. coli-derived mouse FGF acidic/FGF1 protein
Phe16-Asp155, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
4686-FA/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF acidic/FGF1 Protein, CF

  • AFGF
  • alpha
  • alpha-ECGF
  • beta-ECGF
  • ECGF
  • ECGFB
  • ECGF-betaAcidic fibroblast growth factor
  • endothelial cell growth factor, beta
  • FGF acidic
  • FGF-1
  • FGFABeta-endothelial cell growth factor
  • FGF-alpha
  • fibroblast growth factor 1 (acidic)
  • GLIO703
  • HBGF1
  • HBGF-1
  • heparin-binding growth factor 1

Background

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Mouse FGF acidic shares 52% amino acid (aa) sequence identity with FGF basic and 15%-35% with other mouse FGFs. It shares 91%, 96%, 94%, and 100% aa sequence identity with bovine, human, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (4). The secreted FGF acidic is stored in complex with extracellular heparan sulfate (5). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulating FGF acidic bioactivity (6). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (7, 8). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (9). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (10-12). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (13, 14). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (15).

  1. Madiai, F. et al. (1996) Gene 179:231.
  2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
  4. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
  5. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
  6. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
  7. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
  8. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
  9. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
  10. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
  11. Imamura, T. et al. (1990) Science 249:1567.
  12. Wesche, J. et al. (2005) Biochemistry 44:6071.
  13. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
  14. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
  15. Bouleau, S. et al. (2005) Oncogene 24:7839.

Customers Who Viewed This Item Also Viewed...

FGF
basic/FGF2/bFGF [Unco
233-FB
FGF basic/FGF2/bFGF [Unconjugated]
(559 Publications)
Species: Hu
Applications: BA
FGFR1 Antibody
(M19B2) - BSA Free
NB600-1287
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Flow Cytometry FGFR1 Antibody (M19B2) - BSA Free
Western Blot FGFR1 Antibody (M19B2) - BSA Free
(1 Review)
(7 Publications)
Species: Ca, Hu, Mu, Rb, Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, IP, WB
FGFR2 Antibody
(1G3)
H00002263-M01
Immunohistochemistry-Paraffin FGFR2 Antibody (1G3)
Western Blot FGFR2 Antibody (1G3)
Sandwich ELISA FGFR2 Antibody (1G3)
(13 Publications)
Species: Bv, Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, S-ELISA, WB
EGF [Unconjugated]
236-EG
SDS-PAGE EGF [Unconjugated]
Bioactivity EGF [Unconjugated]
Cell Culture EGF [Unconjugated]
(860 Publications)
Species: Hu
Applications: BA
KGF/FGF-7
[Unconjugated]
251-KG
Bioactivity KGF/FGF-7 [Unconjugated]
Bioactivity KGF/FGF-7 [Unconjugated]
(85 Publications)
Species: Hu
Applications: BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
FGF-13 Antibody
NBP2-16471
Immunohistochemistry-Paraffin FGF-13 Antibody
Western Blot FGF-13 Antibody
Immunohistochemistry-Paraffin FGF-13 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
FGF-4
[Unconjugated]
235-F4
SDS-PAGE FGF-4 [Unconjugated]
Bioactivity FGF-4 [Unconjugated]
(76 Publications)
Species: Hu
Applications: BA
HGF [Unconjugated]
294-HG
Bioactivity HGF [Unconjugated]
Cell Culture HGF [Unconjugated]
(114 Publications)
Species: Hu
Applications: BA
IGF-I/IGF-1
[Unconjugated]
291-G1
SDS-PAGE IGF-I/IGF-1 [Unconjugated]
Bioactivity IGF-I/IGF-1 [Unconjugated]
Mass Spectrometry IGF-I/IGF-1 [Unconjugated]
(224 Publications)
Species: Hu
Applications: BA
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(675 Publications)
Species: Mu
Applications: ELISA
FGF-10
[Unconjugated]
345-FG
SDS-PAGE FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
Bioactivity FGF-10 [Unconjugated]
(103 Publications)
Species: Hu
Applications: BA
FGFR3 Antibody
(136312) [Unconjugate ...
MAB7662
Immunohistochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Flow Cytometry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
Immunocytochemistry FGFR3 Antibody (136312) [Unconjugated] - (IIIc)
(1 Review)
(3 Publications)
Species: Hu
Applications: Flow, ICC, IHC, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
FGFR4 Antibody
(240929) [Unconjugate
MAB6852
Immunocytochemistry FGFR4 Antibody (240929) [Unconjugated]
Immunohistochemistry FGFR4 Antibody (240929) [Unconjugated]
Western Blot FGFR4 Antibody (240929) [Unconjugated]
(2 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, IHC, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(820 Publications)
Species: Hu
Applications: BA
FGF-8
[Unconjugated]
423-F8
SDS-PAGE FGF-8 [Unconjugated]
Bioactivity FGF-8 [Unconjugated]
Cell Culture FGF-8 [Unconjugated]
(1 Review)
(63 Publications)
Species: Hu, Mu
Applications: BA

Publications for FGF acidic/FGF1 (4686-FA/CF)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, IP, Stimulation.


Filter By Application
Bioassay
(5)
IP
(1)
Stimulation
(1)
All Applications
Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using 4686-FA/CF Applications Species
F Pei, L Ma, J Jing, J Feng, Y Yuan, T Guo, X Han, TV Ho, J Lei, J He, M Zhang, JF Chen, Y Chai Sensory nerve niche regulates mesenchymal stem cell homeostasis via FGF/mTOR/autophagy axis Nature Communications, 2023-01-20;14(1):344. 2023-01-20 [PMID: 36670126] (Bioassay, Mouse) Bioassay Mouse
K Ichikawa, S Watanabe M, Y Minoshima, J Matsui, Y Funahashi Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy Sci Rep, 2020-02-19;10(1):2939. 2020-02-19 [PMID: 32076044] (IP, Mouse) IP Mouse
J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment Sci Rep, 2019-06-19;9(1):8726. 2019-06-19 [PMID: 31217507] (Bioassay, Mouse) Bioassay Mouse
A Taguchi, R Nishinakam Higher-Order Kidney Organogenesis from Pluripotent Stem Cells Cell Stem Cell, 2017-11-09;0(0):. 2017-11-09 [PMID: 29129523] (Bioassay, Mouse) Bioassay Mouse
Gui T, Sun Y, Gai Z, Shimokado A, Muragaki Y, Zhou G The loss of Trps1 suppresses ureteric bud branching because of the activation of TGF-beta signaling. Dev Biol, 2013-03-26;377(2):415-27. 2013-03-26 [PMID: 23537899] (Mouse) Mouse
Kharitonenkov , Alexei, Beals , John M, Micanovic , Radmila, Strifler , Beth A, Rathnachalam , Radhakri, Wroblewski , Victor J, Li , Shun, Koester , Anja, Ford , Amy M, Coskun , Tamer, Dunbar , James D, Cheng , Christin, Frye , Christop, Bumol , Thomas F, Moller , David E Rational design of a fibroblast growth factor 21-based clinical candidate, LY2405319. PLoS ONE, 2013-03-11;8(3):e58575. 2013-03-11 [PMID: 23536797] (Stimulation, Mouse) Stimulation Mouse
Tucker B, Anfinson K, Mullins R, Stone E, Young M Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med, 2012-12-27;2(1):16-24. 2012-12-27 [PMID: 23283489] (Bioassay, Mouse) Bioassay Mouse
Yamamoto S, Fukumoto E, Yoshizaki K, Iwamoto T, Yamada A, Tanaka K, Suzuki H, Aizawa S, Arakaki M, Yuasa K, Oka K, Chai Y, Nonaka K, Fukumoto S Platelet-derived growth factor receptor regulates salivary gland morphogenesis via fibroblast growth factor expression. J. Biol. Chem., 2008-06-17;283(34):23139-49. 2008-06-17 [PMID: 18559345] (Bioassay, Mouse) Bioassay Mouse

Reviews for FGF acidic/FGF1 (4686-FA/CF) (0)

There are no reviews for FGF acidic/FGF1 (4686-FA/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for FGF acidic/FGF1 (4686-FA/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional FGF acidic/FGF1 Products

Blogs on FGF acidic/FGF1

There are no specific blogs for FGF acidic/FGF1, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse FGF acidic/FGF1 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol Fgf1
Uniprot