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Recombinant Human Syntaxin 4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human Syntaxin 4 Protein, CF Summary

Details of Functionality
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 1-5 μg/mL.
Source
E. coli-derived human Syntaxin 4 protein
Met1-Glu183, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Met1
Protein/Peptide Type
Recombinant Proteins
Gene
STX4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
22.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and TCEP.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Syntaxin 4 Protein, CF

  • NY-REN-31
  • p35-2
  • Renal carcinoma antigen NY-REN-31
  • STX4
  • STX4Ap35-2
  • Syntaxin 4
  • syntaxin 4A (placental)
  • syntaxin-4

Background

Syntaxin 4 (STX4) is a widely expressed type IV transmembrane protein that is a member of the syntaxin family of t‑SNARE (target‑Soluble NSF [N‑ethylmaleimide‑sensitive factor] Attachment REceptor) membrane fusion proteins (1, 2). For membrane fusion, VAMPs (Vesicle‑Associated Membrane Proteins, also called Synaptotagmins) as v‑SNAREs on donor (vesicle) membranes interact with t‑SNAREs and SNAPs (Soluble NSF Attachment Proteins) on target (cell surface) membranes, with SM (Sec1/Munc18-like) accessory proteins acting as catalysts for fusion (2). Human Syntaxin 4 cDNA encodes 297 amino acids (aa) including  basolateral targeting motif for epithelial cells (aa 24‑29), a coiled‑coil domain termed Habc (aa 43‑163), an S‑nitrosylation site which regulates fusion (aa 141), a hinge region, a t‑SNARE domain (aa 200‑262), and a C‑terminal transmembrane domain (aa 276‑296) (1‑4). Within aa 1‑183, human Syntaxin 4 shares 89% aa sequence homology with mouse and rat, and 94‑95% with canine, bovine, equine, porcine, feline and ovine Syntaxin 4. Among its known roles, Syntaxin 4 and SNAP‑23 or SNAP‑25 associate with VAMP‑2 for fusion of GLUT4‑containing vesicles with the plasma membrane in muscle and adipose cells (1, 5). It is required for exocytosis of insulin from pancreatic beta ‑cells (3, 6, 7). With SNAP23 and VAMP‑3, it allows endosome recycling to extend podosomes during macrophage migration (7‑9). It establishes polarization of epithelial cells, specifying basolateral sorting of proteins (4, 10). It mediates postsynaptic activity‑dependent exocytosis in neural cell dendritic spines (11). It is involved in exocytosis of ANP from cardiomyocytes (12). In FAS‑mediated apoptosis, it allows translocation of acid sphingomyelinase to the cell surface (13). Syntaxin 4 binds F‑actin and gelsolin, linking the plasma membrane with, or releasing it from, the actin cytoskeleton (6, 7).

  1. Jagadish, M.N. et al. (1996) Biochem. J. 317:945.
  2. Sudhof, T.C. and J.E. Rothman 2009 Science 323:474.
  3. Wiseman, D.A. et al. (2011) J. Biol. Chem. 286:16344.
  4. Reales, E. et al. (2011) PLoS One 6:e21181.
  5. Vicogne, J. et al. (2006) Proc. Natl. Acad. Sci. USA 103:14761.
  6. Jewell, J.L. et al. (2008) J. Biol. Chem. 283:10716.
  7. Kalwat, M.A. et al. (2012) Mol. Endocrinol. 26:128.
  8. Veale, K.J. et al. (2010) Commun. Integr. Biol. 4:44.
  9. Veale, K.J. et al. (2010) Exp. Cell Res. 317:1817.
  10. Low, S.H. et al. (2006) Mol. Biol. Cell 17:977.
  11. Kennedy, M.J. et al. (2010) Cell 141:524.
  12. Ferlito, M. et al. (2010) J. Mol. Cardiol. 49:791.
  13. Perotta, C. et al. (2010) J. Biol. Chem. 285:40240.

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Bioinformatics

Gene Symbol STX4
Uniprot