Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of CXCL8 is regulated by these truncations, by CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (11). CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Binding Assay, Bioassay.

Showing Publications 1 - 5 of 5.

Publications using 618-IL	Applications	Species
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)	Bioassay	Human
Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells. J. Immunol., 2007-09-01;179(5):3332-41. 2007-09-01 [PMID: 17709550] (Bioassay, Human)	Bioassay	Human
Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression. Int. J. Oncol., 2007-04-01;30(4):977-83. 2007-04-01 [PMID: 17332938] (Bioassay, Human)	Bioassay	Human
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2006-12-29;282(16):11658-66. 2006-12-29 [PMID: 17197447] (Binding Assay, Mouse)	Binding Assay	Mouse
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. J. Cell. Physiol., 2007-02-01;210(2):417-27. 2007-02-01 [PMID: 17096385] (Bioassay, Human)	Bioassay	Human

Array 618-IL

• CXCL8/IL-8 Antibodies
• CXCL8/IL-8 Antibody Pair
• CXCL8/IL-8 ELISA Kits
• CXCL8/IL-8 Kits
• CXCL8/IL-8 Lysate
• CXCL8/IL-8 Proteins
• CXCL8/IL-8 Proteins and Enzymes
• CXCL8/IL-8 ELISAs