| Reactivity | HuSpecies Glossary |
| Applications | Enzyme Activity |
| Format | Carrier-Free |
| Details of Functionality | Measured by the conjugation of reduced glutathione to 1-bromo-2,4-dinitrobenzene. The specific activity is >20,000 pmol/min/μg, as measured under the described conditions. |
| Source | E. coli-derived human Glutathione S-Transferase mu 1/GSTM1 protein Met1-Lys218 |
| Accession # | |
| N-terminal Sequence | Met1 and Pro2 |
| Protein/Peptide Type | Recombinant Enzymes |
| Gene | GSTM1 |
| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
| Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
| Dilutions |
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| Theoretical MW | 26 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE | 25-27 kDa, reducing conditions |
| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol. |
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| Purity | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane |
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| Assay Procedure |
*Adjusted for Substrate Blank **Using the extinction coefficient 9600 M-1cm-1 ***Using the path correction 0.32 cm Note: the output of many spectrophotometers is in mOD. Per Well:
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