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Recombinant Human RCAN3 His Protein

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SDS-Page: Recombinant Human RCAN3 Protein [NBP2-23401]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human RCAN3 His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 56-241 of Human RCAN3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
RCAN3
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol, 0.2 M NaCl
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human RCAN3 His Protein

  • Down syndrome candidate region 1-like 2
  • Down syndrome critical region gene 1-like 2
  • DSCR1L2regulator of calcineurin 3 isoform 13,45,MCIP3regulator of calcineurin 3 isoform 1a2,34,5
  • hRCN3
  • Myocyte-enriched calcineurin-interacting protein 3
  • RCAN family member 3
  • RCN3
  • regulator of calcineurin 3 isoform 1a3,45,Down syndrome candidate region 1-like protein 2
  • regulator of calcineurin 3 isoform 1b2,34,5
  • regulator of calcineurin 3 isoform 1c2,34,5
  • regulator of calcineurin 3 isoform 1c3,45,calcipressin-3
  • Regulator of calcineurin 3

Background

Calcipressin-3, also known as RCAN3, is a 241 amino acid protein that potentially is involved in central nervous system development. RCAN3 inhibits calcineurin-dependent transcriptional responses by binding to the catalytic domain of calcineurin A. Overexpression of calcipressin-3 results in inhibition of calcineurin activity towards the nuclear factor of activated T-cells (NFAT) transcription factors and also downregulates NFAT-dependent cytokine gene expression in activated Jurkat T-cells. Highest expression occurs in heart, skeletal muscle kidney, liver and peripheral blood leukocytes. Recombinant human RCAN3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RCAN3