![Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30158] - Analysis of ABCA1, using NB400-105. 25 ug of whole cell lysate of RAW 264.7 cells either treated with T0901317 (NBP1-30158 lane 1) or untreated (NBP1-30157 lane 2).](http://images.novusbio.com/fullsize/Raw-264.7-Whole-Cell-Lysate-Western-Blot-NBP1-30158-img0003.jpg "Western Blot: Raw 264.7 Whole Cell Lysate [NBP1-30158] - Analysis of ABCA1, using NB400-105. 25 ug of whole cell lysate of RAW 264.7 cells either treated with T0901317 (NBP1-30158 lane 1) or untreated (NBP1-30157 lane 2).")
| Reactivity | MuSpecies Glossary |
| Applications | WB |
| Concentration | 1.0 mg/ml |
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| Application Notes | This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. |
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| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS diluted in 1X lithium dodecyl sulfate (LDS) sample buffer with bromophenol blue and 40-70% glycerol, pH 8.4. BME has not been added. |
| Concentration | 1.0 mg/ml |
| Publication using NBP1-30158 | Applications | Species |
|---|---|---|
| Dong J, Liao W, Tan L et al. Gene Silencing of Receptor-Interacting Protein 2 Protects Against Cigarette Smoke-Induced Acute Lung Injury bioRxiv 2018-10-24 [PMID: 30394320] |
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