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RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

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Western Blot: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RanGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RanGAP1.
Western Blot: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RanGAP1 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.
Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of paraffin-embedded Human liver tissue using anti-RanGAP1 mouse monoclonal antibody.
Flow Cytometry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RanGAP1 ...read more
Western Blot: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.
Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RanGAP1 mouse monoclonal antibody.
Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.
Flow Cytometry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Analysis of Hela cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
OTI1B4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-73797AF350
Alexa Fluor 405
NBP2-73797AF405
Alexa Fluor 488
NBP2-73797AF488
Alexa Fluor 532
NBP2-73797AF532
Alexa Fluor 594
NBP2-73797AF594
Alexa Fluor 647
NBP2-73797AF647
Alexa Fluor 700
NBP2-73797AF700
Alexa Fluor 750
NBP2-73797AF750
Allophycocyanin
NBP2-73797APC
Biotin
NBP2-73797B
CoraFluor 1
NBP2-73797CL1
DyLight 350
NBP2-73797UV
DyLight 405
NBP2-73797V
DyLight 488
NBP2-73797G
DyLight 550
NBP2-73797R
DyLight 594
NBP2-73797DL594
DyLight 650
NBP2-73797C
DyLight 680
NBP2-73797FR
DyLight 755
NBP2-73797IR
FITC
NBP2-73797F
HRP
NBP2-73797H
Janelia Fluor 525
NBP2-73797JF525
Janelia Fluor 549
NBP2-73797JF549
Janelia Fluor 646
NBP2-73797JF646
Janelia Fluor 669
NBP2-73797JF669
PE
NBP2-73797PE
PerCP
NBP2-73797PCP
Unconjugated
NBP2-02623
mFluor Violet 450 SE
NBP2-73797MFV450
mFluor Violet 500 SE
NBP2-73797MFV500
mFluor Violet 610 SE
NBP2-73797MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-02623
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RanGAP1 Antibody (OTI1B4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RANGAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-2000
Theoretical MW
63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

  • Fug1
  • KIAA1835Fug1
  • MGC20266
  • Ran GTPase activating protein 1
  • ran GTPase-activating protein 1
  • RanGAP1
  • SD
  • segregation distorter homolog (Drosophila)
  • segregation distorter homolog
  • segregation distortion

Background

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RANGAP1