![Western Blot: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Western Blot Analysis of THP-1 cell lysate using PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Western-Blot-NBP2-75766-img0005.jpg "Western Blot: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Western Blot Analysis of THP-1 cell lysate using PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | PU1/2146 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75738). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 ) |
| Localization | Nuclear |
| Marker | B-Cell Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | SPI1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Western Blot Ets-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Ets-1_AF7284_Western_Blot_11854.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for PU.1/Spi-1 Antibody (NBP2-75766)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1-Monospecific Mouse MAb (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-75766-img0006.jpg "Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Immunofluorescence Analysis of PFA-fixed Ramos cells labeling PU.1 with PU.1-Monospecific Mouse MAb (PU1/2146) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin (Red).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75766-img0004.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Spleen stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).")
![Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1-Monospecific Mouse MAb (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Flow-Cytometry-NBP2-75766-img0007.jpg "Flow Cytometry: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Flow Cytometric Analysis of PFA-fixed Ramos cells. PU.1-Monospecific Mouse MAb (PU1/2146) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75766-img0001.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75766-img0002.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).")
![Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-75766-img0003.jpg "Immunohistochemistry-Paraffin: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Formalin-fixed, paraffin-embedded human Lymph Node stained with PU.1-Monospecific Mouse Monoclonal Antibody (PU1/2146).")
![Protein Array: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/PU-1-Spi-1-Antibody-PU1-2146-Azide-and-BSA-Free-Protein-Array-NBP2-75766-img0008.jpg "Protein Array: PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free [NBP2-75766] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.")
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