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Pleiotrophin/PTN Antibody (5C3)

 (11 Publications)
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Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of monoclonal antibody to PTN on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.
Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - PTN monoclonal antibody (M01), clone 5C3 Analysis of PTN expression in Hela S3 NE.

Product Details

Summary
Reactivity Hu, Rt, ChSpecies Glossary
Applications WB, ELISA, Func, IHC, IP
Clone
5C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Pleiotrophin/PTN Antibody (5C3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
PTN (NP_002816, 45 a.a. ~ 154 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDCGEWQWSVCVPTSGDCGLGTREGTRTGAECKQTMKTQRCKIPCNWKKQFGAECKYQFQAWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCGKLTKPKPQAESK
Specificity
PTN - pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTN
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using
H00005764-M01 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pleiotrophin/PTN Antibody (5C3)

  • HARP
  • HBBM
  • HB-GAM
  • HBGF8
  • HBNF
  • HBNF1
  • HBNF-1
  • Heparin-binding brain mitogen
  • HNGF-8
  • NEGF1HBGF-8
  • neurite growth-promoting factor 1
  • neurite growth-promoting factor1)
  • OSF-1
  • Osteoblast-specific factor 1
  • Pleiotrophin
  • PTN

Background

Heparin binding mitogenic protein. Has neurite extension activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Pleiotrophin/PTN Antibody (H00005764-M01)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
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Human
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using H00005764-M01 Applications Species
Mengmeng Zhang, Kaiwen Zhou, Zilin Wang, Ting Liu, Laura E. Stevens, Filipa Lynce, Wendy Y. Chen, Sui Peng, Yubin Xie, Duanyang Zhai, Qianjun Chen, Yawei Shi, Huijuan Shi, Zhongyu Yuan, Xiaoping Li, Juan Xu, Zhenhai Cai, Jianping Guo, Nan Shao, Ying Lin A Subpopulation of Luminal Progenitors Secretes Pleiotrophin to Promote Angiogenesis and Metastasis in Inflammatory Breast Cancer Cancer Research 2024-06-04 [PMID: 38507720]
Zhu Q, Tan M, Wang C et al. Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos Cell & bioscience 2023-07-19 [PMID: 37468984] (IHC, Human) IHC Human
Margarita L, Marina K, Angelos K et al. Binding of pleiotrophin to cell surface nucleolin mediates prostate cancer cell adhesion to osteoblasts. Tissue Cell. 2022-04-16 [PMID: 35461017]
Margarita L, Pinelopi K, Fani K et al. Pleiotrophin selectively binds to vascular endothelial growth factor receptor 2 and inhibits or stimulates cell migration depending on 3 integrin expression. Angiogenesis. 2020-07-17 [PMID: 32681389]
Kaspiris A, Mikelis C, Heroult M et al. Expression of the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the serum, cartilage and subchondral bone of patients with osteoarthritis. Joint Bone Spine. 2013-01-17 [PMID: 23333521]
Koutsioumpa M, Polytarchou C, Courty J et al. Interplay between alphavb3 Integrin and Nucleolin Regulates Human Endothelial and Glioma Cell Migration. J Biol Chem. 2012-11-16 [PMID: 23161541]
Miao J, Ding M, Zhang A et al. Pleiotrophin promotes microglia proliferation and secretion of neurotrophic factors by activating extracellular signal-regulated kinase 1/2 pathway. Neurosci Res. 2012-09-18 [PMID: 23000062]
Tsirmoula S, Dimas K, Hatziapostolou M et al. Implications of pleiotrophin in human PC3 prostate cancer cell growth in vivo. Cancer Sci. 2012-08-14 [PMID: 22783964]
Mikelis C, Lamprou M, Koutsioumpa M et al. A peptide corresponding to the C-terminal region of pleiotrophin inhibits angiogenesis in vivo and in vitro. J Cell Biochem. 2011-02-22 [PMID: 21344482]
Mikelis C, Sfaelou E, Koutsioumpa M et al. Integrin {alpha}{nu}{beta}3 is a pleiotrophin receptor required for pleiotrophin-induced endothelial cell migration through receptor protein tyrosine phosphatase {beta}/{zeta}. FASEB J. 2009-01-13 [PMID: 19141530]
Show All 11 Publications.

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Bioinformatics

Gene Symbol PTN
Entrez
OMIM
Uniprot