![Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)](http://images.novusbio.com/fullsize/Phosphoserine-Antibody-PSR-45-Western-Blot-NB600-405-img0003.jpg "Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)")
| Reactivity | Hu, Rt, RbSpecies Glossary |
| Applications | WB, ELISA |
| Clone | PSR-45 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Specificity | By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. The ELISA dilution given is for indirect. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Ascites |
| Preservative | 0.09% Sodium Azide |
| Purity | Unpurified |
| Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Verified Customer |
ELISA | Mouse | 07/24/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Phosphoserine Antibody (NB600-405)Find related products by research area.
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Sample collection from mammalian culture cells for kinomic analysis By Jamshed Arslan Pharm.D., PhD.IntroductionKinome describes kinases, and kinomics refers to the kinase signaling. Studying the effects of reagent (exogenously applied growth factor or inhibitor) on kinase activit... Read full blog post. |
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Phosphotyrosine: A Global Player in Human Health and Disease Protein tyrosine phosphorylation is a fundamental mechanism for controlling many aspects of cellular processes, as well as aspects of human health and diseases. Compared with phosphoserine and phosphothreonine, phosphotyrosine signaling is more tightl... Read full blog post. |
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New Techniques Using Phosphoserine Antibodies Phosphoserine, the phosphorylated modification of the amino acid serine, is a central post-translational modification within a cell for many biological and biomedical processes. The phosphorylation of specifically four residue types - histidine, serin... Read full blog post. |
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Phosphotyrosine: Affecting Locally Acting Globally Over the past two decades, it has become clear that tyrosine phosphorylation plays a pivotal role in a variety of important signaling pathways in multicellular organisms. In the typical vertebrate cell, phosphotyrosine represents only a tiny fraction ... Read full blog post. |
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Phosphoserine: A Basic Cellular Signal with Wide Ranging Effects Phosphoserine is an ester of serine and phosphoric acid which results from posttranslational modifications. Phosphorylation is a key post-translational modification necessary for normal cellular signaling. Phosphorylated proteins mediate cell division... Read full blog post. |
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Phosphotyrosine is Critical Signal Transduction and Regulation Phosphotyrosine is the phosphorylated version of the amino acid tyrosine, which results from the activation of intracellular protein kinases (e.g. via growth factors) during normal growth and development, well as in transformation and oncogenesis. Pho... Read full blog post. |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")