| Reactivity | All-MultiSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 179003 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Alkaline Phosphatase |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Agarose | AGM1676 | |||
| Biotin | BAM1676 | |||
| HRP | HAM1676 | |||
| Unconjugated | MAB1676-100 | |||
| Specificity | Detects proteins containing phosphorylated Tyrosine residues. ELISA and 2D Western blot analyses using pervanadate-treated cell lysates indicate that it binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. This antibody does not cross-react with proteins or peptides containing phosphorylated serine or threonine residues. |
| Source | N/A |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
| Buffer | Supplied as a 0.2 μm filtered solution in phosphate-buffered saline (PBS) with stabilizers
and preservatives. |
| Preservative | No Preservative |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.5 mg/mL. |
Secondary Antibodies |
Isotype Controls |
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![Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.](http://images.novusbio.com/fullsize2/Tyrosine_APM1676_Western_Blot_5944.jpg "Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1:1000 dilution of Phospho-Tyrosine AP-conjugated Monoclonal Antibody (Catalog # APM1676), followed by [Secondary Antibody Proper Name & Link]. Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group XXX.")