![Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-34497-img0004.jpg "Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.")
| Reactivity | MuSpecies Glossary |
| Applications | Flow, Func, ICC/IF, CyTOF-ready |
| Clone | J43 |
| Clonality | Monoclonal |
| Host | Armenian Hamster |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | The immunogen for this antibody was PD1. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Armenian Hamster |
| Gene | PDCD1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | The J43 antibody has been tested by flow cytometric analysis of mouse Con-A activated spleen cells and mouse PD-1 transfected cells. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PDCD1 |
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0005.jpg "Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig")
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0001.jpg "Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.")
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0003.jpg "Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.")
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0002.jpg "Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells")
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