![Immunocytochemistry/Immunofluorescence: PCOLCE2 Antibody [NBP1-80935] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/PCOLCE2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80935-img0003.jpg "Immunocytochemistry/Immunofluorescence: PCOLCE2 Antibody [NBP1-80935] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: PEGKVVVLNFRFIDLESDNLCRYDFVDVYNGHANGQRIGRFCGTFRPGALVSSGNKMMVQMISDANTAGNGFMAMFSAAEPNERGDQYCGGLLDRPSGSFKTPNWPDRDYPAGVTCVWHIVAPKNQLIELKFEKFDVERDNY |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PCOLCE2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
![Immunohistochemistry FoxN1 Antibody (717008) [Unconjugated]](https://images.novusbio.com/images2/FoxN1_MAB7295_Immunohistochemistry_11873.jpg)
| Publication using NBP1-80935 | Applications | Species |
|---|---|---|
| Ju W, Greene CS, Eichinger F et al. Defining cell-type specificity at the transcriptional level in human disease. Genome Res 2013-11-01 [PMID: 23950145] |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PCOLCE2-Antibody-Immunohistochemistry-Paraffin-NBP1-80935-img0008.jpg "Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/PCOLCE2-Antibody-Immunohistochemistry-Paraffin-NBP1-80935-img0005.jpg "Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human kidney shows strong cytoplasmic/membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/PCOLCE2-Antibody-Immunohistochemistry-Paraffin-NBP1-80935-img0006.jpg "Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining of human kidney shows strong cytoplasmic/membranous positivity in cells in glomeruli.")
![Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/PCOLCE2-Antibody-Immunohistochemistry-Paraffin-NBP1-80935-img0007.jpg "Immunohistochemistry-Paraffin: PCOLCE2 Antibody [NBP1-80935] - Staining shows moderate cytoplasmic positivity in smooth muscle cells.")
![PCPE-1/PCOLCE Antibody [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "PCPE-1/PCOLCE Antibody [Unconjugated]")
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21018.jpg)
![Western Blot BMP-1/PCP Antibody [Unconjugated]](https://images.novusbio.com/images2/BMP1_AF1927_Western_Blot_21019.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")