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Orexin B/Hypocretin-2 Antibody (145202)

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Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the neuronal processes and synaptic boutons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Orexin B was detected in perfusion fixed frozen sections of mouse brain (hypothalamus) using Mouse Anti-Human Orexin B Monoclonal Antibody (Catalog # MAB734) at 8 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Orexin B, p38 alpha , and Integrin beta 1 were detected in perfusion fixed frozen sections of mouse brainstem using Mouse Anti-Human Orexin B Monoclonal Antibody (red; Catalog # MAB734), Anti-Human/Mouse/Rat p38 alpha ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
145202
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB734-SP
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Orexin B/Hypocretin-2 Antibody (145202) Summary

Immunogen
KLH-conjugated human Orexin B/Hypocretin‑2 synthetic peptide
Accession # O43612
Specificity
Detects human Orexin B/Hypocretin‑2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Orexin A is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HCRT
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB734 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Orexin B/Hypocretin-2 Antibody (145202)

  • Hcrt 2
  • HCRT
  • Hcrt2
  • Hypocretin (orexin) neuropeptide precursor
  • Hypocretin 2
  • Hypocretin B
  • Hypocretin precursor
  • Hypocretin
  • Hypocretin2
  • Hypocretin-2
  • Orexin B
  • Orexin neuropeptide precursor
  • Orexin precursor
  • Orexin
  • OrexinB
  • PPORX
  • PPOX

Background

Orexin A and Orexin B are neuropeptide hormones derived from proteolytic processing of the Orexin precursor protein (Accession # O43612). Orexins are released from the hypothalamus and are involved in the regulation of sleeping patterns, feeding, and metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Orexin B/Hypocretin-2 Antibody (145202)
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Publications for Orexin B/Hypocretin-2 Antibody (MAB734)(3)

We have publications tested in 1 confirmed species: Alpaca.

We have publications tested in 1 application: IHC.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HCRT
Uniprot