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Human O-GlcNAcase/OGA/MGEA5 ELISA Kit (Colorimetric)

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ELISA: Human O-GlcNAcase/OGA/MGEA5 ELISA Kit (Colorimetric) [NBP3-39071] - Standard Curve Reference
ELISA: Human O-GlcNAcase/OGA/MGEA5 ELISA Kit (Colorimetric) [NBP3-39071] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Urine
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.55 ng/mL (example only; lot dependent)

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Human O-GlcNAcase/OGA/MGEA5 ELISA Kit (Colorimetric) Summary

Description
Assay Length: 4.5 hours
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.55 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
OGA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Reagent A
  2. Detection Reagent B
  3. Diluent Buffer
  4. Instruction manual
  5. Plate sealer for 96 wells
  6. Pre-coated 96T strip plate
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human O-GlcNAcase/OGA/MGEA5 ELISA Kit (Colorimetric)

  • bifunctional protein NCOAT
  • FLJ11229
  • GH84
  • HEXC
  • HEXC3
  • Hexosaminidase B
  • hyaluronidase in meningioma
  • KIAA0679
  • MEA5FLJ23355
  • meningioma expressed antigen 5 (hyaluronidase)
  • Meningioma-expressed antigen 5
  • MGEA5
  • NCOAT
  • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
  • OGA
  • OGlcNAcase
  • O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol OGA