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O-GlcNAcase/OGA/MGEA5 Antibody (9B9)

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Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (9B9) [NBP3-42998] - Human brain lysate were subjected to SDS PAGE followed by western blot with (O-GlcNAcase/OGA/MGEA5 antibody) at dilution of 1:600
Western Blot: O-GlcNAcase/OGA/MGEA5 Antibody (9B9) [NBP3-42998] - Human brain lysate were subjected to SDS PAGE followed by western blot with (O-GlcNAcase/OGA/MGEA5 antibody) at dilution of 1:600
Immunohistochemistry: O-GlcNAcase/OGA/MGEA5 Antibody (9B9) [NBP3-42998] - Immunohistochemistry of paraffin-embedded human gliomas using (O-GlcNAcase/OGA/MGEA5 antibody) at dilution of 1:50

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
9B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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O-GlcNAcase/OGA/MGEA5 Antibody (9B9) Summary

Immunogen
meningioma expressed antigen 5(hyaluronidase) - (Uniprot# O60502)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
OGA
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Western Blot 1:500 - 1:1000
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for O-GlcNAcase/OGA/MGEA5 Antibody (9B9)

  • bifunctional protein NCOAT
  • FLJ11229
  • GH84
  • HEXC
  • HEXC3
  • Hexosaminidase B
  • hyaluronidase in meningioma
  • KIAA0679
  • MEA5FLJ23355
  • meningioma expressed antigen 5 (hyaluronidase)
  • Meningioma-expressed antigen 5
  • MGEA5
  • NCOAT
  • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
  • OGA
  • OGlcNAcase
  • O-GlcNAcase

Background

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol OGA