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O-GlcNAc Antibody (RL2) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, Fi, Ha, Pm, V-Vi, XpSpecies Glossary
Applications WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IP, ChIP
Clone
RL2
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

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O-GlcNAc Antibody (RL2) [DyLight 350] Summary

Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Localization
Cytoplasmic and Nuclear
Specificity
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for O-GlcNAc Antibody (RL2) [DyLight 350]

  • GlcNAc
  • O-linked N-acetylglucosamine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for O-GlcNAc Antibody (NB300-524UV) (0)

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Secondary Antibodies

 

Isotype Controls

Additional O-GlcNAc Products

Array NB300-524UV

Research Areas for O-GlcNAc Antibody (NB300-524UV)

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Blogs on O-GlcNAc.

O-GlcNAc, Glucose Deprivation and Cancer
O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin...  Read full blog post.

mFluor Violet Conjugated Antibodies

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