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O-GlcNAc Antibody (RL2) [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Dr, Fi, Ha, Pm, V-Vi, XpSpecies Glossary
Applications WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IP, ChIP
Clone
RL2
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 405

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O-GlcNAc Antibody (RL2) [Alexa Fluor® 405] Summary

Immunogen
Pore complex-lamina fraction purified from rat liver nuclear envelopes.
Localization
Cytoplasmic and Nuclear
Specificity
Detects nuclear pore complex (NPC), cytoplasmic and intranuclear O-linked glycoproteins from human, mouse, and rat tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 26004176). Xenopus reactivity reported in scientific literature (PMID: 17329255). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for O-GlcNAc Antibody (RL2) [Alexa Fluor® 405]

  • GlcNAc
  • O-linked N-acetylglucosamine

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for O-GlcNAc Antibody (NB300-524AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Additional O-GlcNAc Products

Array NB300-524AF405

Research Areas for O-GlcNAc Antibody (NB300-524AF405)

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Blogs on O-GlcNAc.

O-GlcNAc, Glucose Deprivation and Cancer
O-linked beta-N-acetylglucosamine (O-GlcNAc) is a sugar attachment to serine or threonine hydroxyl moieties on nuclear and cytoplasmic proteins. O-GlcNAc modified proteins are generally either cytoplasmic or nuclear proteins, and unlike asparagine-lin...  Read full blog post.

mFluor Violet Conjugated Antibodies

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