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Recombinant Human NT-proBNP Protein

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SDS-Page: Recombinant Human NT-proBNP Protein [NBP2-61321] - Recombinant Human NT-proBNP Protein.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
LYOPH

Order Details

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Recombinant Human NT-proBNP Protein Summary

Description
A single non-glycosylated polypeptide chain containing 76 amino acids corresponding to NT-proBNP Source: E. coli

Uniprot ID: P16860

Amino Acid Sequence: HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR

Details of Functionality
Data is not available.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NPPB
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of NT-proBNP as determined by LAL method.

Applications/Dilutions

Dilutions
  • SDS-Page
  • Western Blot
Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl.
Preservative
No Preservative
Concentration
LYOPH
Purity
>98%, by SDS-PAGE and HPLC
Reconstitution Instructions
Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Alternate Names for Recombinant Human NT-proBNP Protein

  • BNP
  • brain type natriuretic peptide
  • Gamma-brain natriuretic peptide
  • natriuretic peptide precursor B
  • natriuretic peptides B
  • natriuretic protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol NPPB