![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0009.jpg "Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant NT-proBNP expressed in E. coli in Western blotting by different monoclonal antibodies after Tricine- SDS gel electrophoresis. Lanes: 1 - MAb 5B61-12; 2 - 15F1113-24; 3 - 11D131-39; 4 - 15D748-56; 5 - 24E1167-76. Antigen: recombinant NT-proBNP expressed in E. coli, 2.5 ug/well.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 24E11cc |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic NT-proBNP, corresponding to a.a.r. 61-76, conjugated with carrier protein. |
| Epitope | a.a.r. 67-76 |
| Localization | Secreted |
| Specificity | Human NT-proBNP and proBNP |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | NPPB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards. Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0011.jpg "Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Tricine-SDS-PAGE in reducing conditions of recombinant NT-proBNP and proBNP expressed in E. coli. Lane 1: low molecular weight standards. Lane 2: proBNP, 3 ug Lane 3: NT-proBNP, 3 ug Gel staining: Coomassie brilliant blue R-250.")
![Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 7, 8, 9 and 10 do not recognize glycosylated proBNP expressed in the mammalian cell line.](http://images.novusbio.com/fullsize/proBNP-Antibody-24E11-Western-Blot-NB120-13123-img0010.jpg "Western Blot: NT-proBNP Antibody (24E11) [NB120-13123] - Detection of human recombinant proBNP expressed in mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells 0.15 ug/line. MAbs 7, 8, 9 and 10 do not recognize glycosylated proBNP expressed in the mammalian cell line.")
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